Probe:	OG-12 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present		2Aa	Expression starts at the onset of limb outgrowth.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Widespread	2Ab	Broadly expressed throughout the whole limb buds.
TS17: hindlimb bud	Present	Widespread	2Ab	Broadly expressed throughout the whole limb buds.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	2Ac	Expressed at the proximal part.
TS19: hindlimb bud	Present	Regionally restricted	2Ac	Expressed at the proximal part.

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	2Ad	Expressed at the proximal part.
TS20: hindlimb	Present	Regionally restricted	2Ad	Expressed at the proximal part.

Structure	Level	Pattern	Image	Note
TS21: forelimb	Present	Regionally restricted	2Ae	Expressed at the proximal part.
TS21: hindlimb	Present	Regionally restricted	2Ae	Expressed at the proximal part.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Widespread	2Ba	Expression overlaps with the broader of Tbx4 expression domain.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud mesenchyme	Present	Regionally restricted	2Ba'	Expression broadly overlaps with Tbx4 throughout the proximal limb mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Present	Regionally restricted	2Bd	Expressed at the proximal part. Expression patterns of Shox2 and Tbx4 are mutually exclusive.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	2Bd'	Expression broadly overlaps with Tbx4 throughout the proximal limb mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	2Bg	Expressed at the proximal part. Expression patterns of Shox2 and Tbx4 are mutually exclusive.

Structure	Level	Pattern	Image	Note
TS20: hindlimb mesenchyme	Present	Regionally restricted	2Bg'	Expressed in areas surrounding the proximal cartilaginous elements.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		4Aa , 4Ac
TS17: hindlimb bud	Present		4Aa , 4Ae

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		4Ab , 4Ad	Increased expression compared with the wild-type.
TS17: hindlimb bud	Present		4Ab , 4Af	Increased expression compared with the wild-type.