Gene Expression Data
Assay Details
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Assay
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Reference:
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J:239256
Zheng L, et al., Runx2/DICER/miRNA Pathway in Regulating Osteogenesis. J Cell Physiol. 2017 Jan;232(1):182-91
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5912292
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Gene symbol:
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Dicer1
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Gene name:
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dicer 1, ribonuclease type III
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Antibody:
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anti-Dicer
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
4Aa:
embryonic day 16.5
(more  )
Specimen
4Aa:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: femur |
Present |
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4Aa2
,
4Aa1
,
4Aa3
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Specimen
4Ab:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Runx2tm1Mjo/Runx2tm1Mjo
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: femur |
Present |
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4Ab3
,
4Ab1
,
4Ab2
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Levels of expression are lower in the mutant compared to wild type.
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Specimen
4Ac:
embryonic day 16.5
(more )
Specimen
4Ac:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: lower jaw mesenchyme |
Present |
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4Ac1
,
4Ac3
,
4Ac2
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Expression is present in the mandible mesenchyme that surrounds Meckel's cartilage.
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Specimen
4Ad:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Runx2tm1Mjo/Runx2tm1Mjo
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
10% Formalin
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Embedding:
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Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: lower jaw mesenchyme |
Present |
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4Ad3
,
4Ad2
,
4Ad1
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Expression is present in the mandible mesenchyme that surrounds Meckel's cartilage. Levels of expression are lower in the mutant compared to wild type.
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Specimen
4B -/-:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Runx2tm1Mjo/Runx2tm1Mjo
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
|
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: embryo |
Present |
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4B -/-
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Levels of expression are lower in the mutant compared to wild type.
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Specimen
4B +/-:
embryonic day 16.5; Runx2tm1Mjo/Runx2 +
(more )
Specimen
4B +/-:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Runx2tm1Mjo/Runx2+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
|
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: embryo |
Present |
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4B +/-
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Specimen
4B +/+:
embryonic day 16.5
(more )
Specimen
4B +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
|
embryonic day 16.5
|
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: embryo |
Present |
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4B wt/wt
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Analysis Tools