Epha4 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:242459 Huettl RE, et al., Engrailed 1 mediates correct formation of limb innervation through two distinct mechanisms. PLoS One. 2015;10(2):e0118505
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5912247
Gene symbol:	Epha4
Gene name:	Eph receptor A4

Antibody:	Anti-Epha4
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

2c WT ©

2c WT merge ©

Specimen 2c wildtype: embryonic day 12.5 (more

)
Note: Double labeled: green - Epha4; red - Nefm (assay MGI:5912250 and Nefh (assay MGI:5912403).

Structure	Level	Pattern	Image	Note
TS20: brachial plexus	Present	Regionally restricted	2c WT merge , 2c WT	Largely restricted to the dorsally projecting branch.

2c Mut ©

2c Mut merge ©

Specimen 2c En1-/-: embryonic day 12.5; En1^tm2Alj/En1^tm2Alj (more

)
Note: Double labeled: green - Epha4; red - Nefm (assay MGI:5912250 and Nefh (assay MGI:5912403).

Structure	Level	Pattern	Image	Note
TS20: brachial plexus	Present		2c Mut merge , 2c Mut	Expressed in the axons in dorsal and ventral branches.

S1a left ©

Specimen S1a wildtype: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present	Regionally restricted	S1a left	Mainly found in dorsal area. Small sites of ventral expression were seen.

S1a right ©

Specimen S1a En1-/-: embryonic day 12.5; En1^tm2Alj/En1^tm2Alj (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present	Regionally restricted	S1a right	Mainly found in dorsal area. Small sites of ventral expression were seen.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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