Nefm Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:242459 Huettl RE, et al., Engrailed 1 mediates correct formation of limb innervation through two distinct mechanisms. PLoS One. 2015;10(2):e0118505
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5912250
Gene symbol:	Nefm
Gene name:	neurofilament, medium polypeptide

Antibody:	anti-Neurofilament 3H5
Detection system:	Secondary antibody coupled to Alexa Fluor
Assay notes:	Antibody recognizes both Nefm and Nefh.

Results

1a Lmx1b WT ©

Specimen 1a wildtype: embryonic day 10.5 (more

)
Note: Double labeled: red - Nefh and Nefm; gray - Lmx1b (assay MGI:5912227).

Structure	Level	Pattern	Image	Note
TS17: spinal nerve plexus	Present		1a Lmx1b WT	Expressed in the axons.

1a Lmx1b Mut ©

Specimen 1a En1-/-: embryonic day 10.5; En1^tm2Alj/En1^tm2Alj (more

)
Note: Double labeled: red - Nefh and Nefm; gray - Lmx1b (assay MGI:5912227).

Structure	Level	Pattern	Image	Note
TS17: spinal nerve plexus	Present		1a Lmx1b Mut	Expressed in the axons.

1b Lmx1b WT ©

Specimen 1b wildtype: embryonic day 11.5 (more

)
Note: Double labeled: red - Nefh and Nefm; gray - Lmx1b (assay MGI:5912227).

Structure	Level	Pattern	Image	Note
TS19: brachial plexus	Present		1b Lmx1b WT	Expressed in the axons, which have split into dorsal and ventral branches.

1b Lmx1b Mut ©

Specimen 1b En1-/-: embryonic day 11.5; En1^tm2Alj/En1^tm2Alj (more

)
Note: Double labeled: red - Nefh and Nefm; gray - Lmx1b (assay MGI:5912227).

Structure	Level	Pattern	Image	Note
TS19: brachial plexus	Present		1b Lmx1b Mut	Expressed in the axons, which have split into dorsal and ventral branches.

1c right ©

Specimen 1c: embryonic day 10.5 (more

)
Note: Double labeled: red - Nefh and Nefm; green - En1 (assay MGI:5912246).

Structure	Level	Pattern	Image	Note
TS17: spinal nerve plexus	Present	Regionally restricted	1c right	Expressed in the axons.

1d right ©

Specimen 1d: embryonic day 11.5 (more

)
Note: Double labeled: red - Nefh and Nefm; green - En1 (assay MGI:5912246).

Structure	Level	Pattern	Image	Note
TS19: brachial plexus	Present		1d right	Expressed in the axons, which have split into dorsal and ventral branches.

2c WT merge ©

Specimen 2c wildtype: embryonic day 12.5 (more

)
Note: Double labeled: red - Nefh and Nefm; green - Epha4 (assay MGI:5912247).

Structure	Level	Pattern	Image	Note
TS20: brachial plexus	Present		2c WT merge	Expressed in the axons in dorsal and ventral branches.

2c Mut merge ©

Specimen 2c En1-/-: embryonic day 12.5; En1^tm2Alj/En1^tm2Alj (more

)
Note: Double labeled: red - Nefh and Nefm; green - Epha4 (assay MGI:5912247).

Structure	Level	Pattern	Image	Note
TS20: brachial plexus	Present		2c Mut merge	Expressed in the axons in dorsal and ventral branches.

S1b left ©

Specimen S1b wildtype: embryonic day 12.5 (more

)
Note: Double labeled: red - Nefh and Nefm; green - Epha7 (assay MGI:5912249).

Structure	Level	Pattern	Image	Note
TS20: brachial plexus	Present		S1b left

S1b right ©

Specimen S1b En1-/-: embryonic day 12.5; En1^tm2Alj/En1^tm2Alj (more

)
Note: Double labeled: red - Nefh and Nefm; green - Epha7 (assay MGI:5912249).

Structure	Level	Pattern	Image	Note
TS20: brachial plexus	Present		S1b right	Expressed in the axons, which have split into dorsal and ventral branches.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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