Antibody:	Anti-Zac1
Detection system:	Secondary antibody coupled to Cy3
Assay notes:	Fixative was 4% paraformaldehyde/0.02% glutaraldehyde with a post-fix in 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS26: cerebellum external granule cell layer	Present	Regionally restricted	2H
TS26: cerebellum layer	Present	Single cells	2H	Expression is in the internal granule layer.
TS26: cerebellum Purkinje cell cluster	Present	Single cells	2H

Structure	Level	Pattern	Image	Note
TS27: cerebellum granule cell layer	Present		2I
TS27: cerebellum external granule cell layer	Absent		2I

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Present		2J
TS28: cerebellum Purkinje cell layer	Present	Single cells	2J	Expression is in a subset of Purkinje cells located in the posterior cerebellum (lobules IV to X).

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Absent		2K
TS28: cerebellum internal granule cell layer	Absent		2K

Structure	Level	Pattern	Image	Note
TS26: cerebellar cortex	Present	Regionally restricted	2M	Expression is in cells migrating from the outer layer of the external granule layer into the immature molecular layer.
TS26: cerebellum external granule cell layer	Present	Regionally restricted	2M	Expression is in cells migrating from the outer layer of the external granule layer into the immature molecular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present	Regionally restricted	3C , 3A	Expression is in the granule layer of the posterior lobules.
TS28: cerebellum vermis lobule	Present	Regionally restricted	3C , 3A	Expression is in the granule layer of the posterior lobules.

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present	Regionally restricted	3G , 3I	Expression is in the granule layer of the posterior lobules.
TS28: cerebellum vermis lobule	Present	Regionally restricted	3G , 3I	Expression is in the granule layer of the posterior lobules.