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Gene Expression Data
Assay Details
Assay
Reference: J:173393 Chung SH, et al., Zac1 plays a key role in the development of specific neuronal subsets in the mouse cerebellum. Neural Dev. 2011;6:25
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5912025
Gene symbol: Calb1
Gene name: calbindin 1
Antibody: anti-calbindin D-28K (#300)
Detection system: Secondary antibody coupled to Cy2
Assay notes: Fixative was 4% paraformaldehyde/0.02% glutaraldehyde with a post-fix in 4% paraformaldehyde.
Results
Specimen 2H: embryonic day 18.0 (more )
Note: Double labeled: green - Calb1; red - Plagl1 (assay MGI:5912040).
Structure Level Pattern Image Note
TS26: cerebellum Purkinje cell cluster Present 2H
Specimen 2I: postnatal day 0 (more )
Note: Double labeled: green - Calb1; red - Plagl1 (assay MGI:5912040).
Structure Level Pattern Image Note
TS27: cerebellum Purkinje cell cluster Present 2I
Specimen 2J: postnatal day 8 (more )
Note: Double labeled: green - Calb1; red - Plagl1 (assay MGI:5912040).
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Present 2J
Specimen 2K: postnatal day 20 (more )
Note: Double labeled: green - Calb1; red - Plagl1 (assay MGI:5912040).
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Present 2K
Specimen not shown WT: postnatal adult (more )
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Present
Specimen not shown Zac1+m/-: postnatal adult; Plagl1tm1Jour/Plagl1+ (more )
Note: Zac1+m/- heterozygotes are effectively Zac1 null mutants due to maternal imprinting of the Plagl1 locus.
Structure Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Present Expression in this mutant is the same as in wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory