Gene Expression Data
Assay Details
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Assay
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Reference:
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J:208266
Dong F, et al., Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm. J Cell Biol. 2014 Jan 20;204(2):203-13
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5911992
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Gene symbol:
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Dnaaf6rt
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Gene name:
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dynein axonemal assembly factor 6, retrotransposed
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Antibody:
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anti-Pih1d3
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
4A upper:
postnatal adult
(more  )
Specimen
4A upper:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatid |
Present |
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4A upper
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Localized to the cytoplasm of round spermatids. Not detected in elongating or elongated spermatids.
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| TS28: spermatocyte |
Present |
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4A upper
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Localized to the cytoplasm. Highest expression at the diplotene and pachytene stages.
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Specimen
4A middle:
postnatal adult
(more )
Specimen
4A middle:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatid |
Present |
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4A middle
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Localized to the cytoplasm of round spermatids. Not detected in elongating or elongated spermatids.
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| TS28: spermatocyte |
Present |
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4A middle
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Highest expression at the diplotene and pachytene stages.
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Specimen
4A lower:
(close )
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Genetic Background:
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involves: ICR
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Mutant Allele(s):
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Dnaaf6rttm1.1Hmd/Dnaaf6rttm1.1Hmd
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools