Antibody:	PS-2
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	The authors did not specify which rat anti-mouse antibody was used, either this or the R1-2 antibody (MGI:5911485), or the anti-CD49d (MGI:5911483) antibody may have been used.

Structure	Level	Pattern	Image	Note
TS21: trunk musculature	Present	Regionally restricted	1a	Expression is in muscle forming regions between the ribs.

Structure	Level	Pattern	Image	Note
TS21: vertebral axis musculature	Present	Regionally restricted	1b	Expression is in spinal muscles around the vertebrae.
TS21: diaphragm	Present		1b

Structure	Level	Pattern	Image	Note
TS21: tongue muscle	Present		1c	Expression is in the horizontal and vertical muscle layers.

Structure	Level	Pattern	Image	Note
TS23: back musculature	Present		1d

Structure	Level	Pattern	Image	Note
TS24: arterial system smooth muscle	Present		2a' , 2a	Expression is in the aorta.
TS24: blood vessel endothelium	Weak		2a' , 2a	Expression is in the aorta.

Structure	Level	Pattern	Image	Note
TS23: blood vessel endothelium	Present	Regionally restricted	2c' , 2c	Expression is in vascular smooth muscle.
TS23: lung vascular element	Present	Regionally restricted	2c' , 2c	Expression is in vascular smooth muscle.
TS23: smooth muscle tissue	Present	Regionally restricted	2c' , 2c	Expression is in vascular smooth muscle.
TS23: blood vessel	Present	Regionally restricted	2c' , 2c	Expression is in the mesenchyme surrounding the smooth muscle.

Structure	Level	Pattern	Image	Note
TS23: lung vascular element	Present	Regionally restricted	2d , 2d'	Expression is in vascular smooth muscle.
TS23: lung	Present	Regionally restricted	2d' , 2d	Expression is in vascular smooth muscle and absent in airway smooth muscle.

Structure	Level	Pattern	Image	Note
TS28: intestine lamina propria	Present		3a' , 3a
TS28: small intestine smooth muscle	Present		3a' , 3a

Structure	Level	Pattern	Image	Note
TS21: esophagus	Present	Regionally restricted	3c , 3c'	Expression is on the muscularis smooth muscle layer that surrounds the submucosa.

Structure	Level	Pattern	Image	Note
TS21: trachea vascular element	Present		3d
TS21: lower respiratory tract	Present	Regionally restricted	3d	Expression is in smooth muscle surrounding the trachea.

Structure	Level	Pattern	Image	Note
TS28: stomach smooth muscle	Present	Regionally restricted	3e	Expression is as streaks along the surface.
TS28: stomach	Present	Single cells	3e	Expression is in the adventitia.

Structure	Level	Pattern	Image	Note
TS21: outflow tract	Present	Regionally restricted	4a' , 4a	Expression is on endocardial cusions forming the conus septum of the outflow tract and between the pulmonary artery and aorta.
TS21: visceral pericardium	Present		4a' , 4a
TS21: dorsal aorta	Present	Regionally restricted	4a , 4a'	Expression is on cusps.
TS21: pulmonary artery	Present	Regionally restricted	4a , 4a'	Expression is on cusps.
TS21: atrioventricular endocardial cushion	Weak		4a , 4a'

Structure	Level	Pattern	Image	Note
TS21: liver	Present	Single cells	5a , 5a'	Expression is in immune cells.

Structure	Level	Pattern	Image	Note
TS28: spleen red pulp	Present	Regionally restricted	5c' , 5c	Expression is in loosely packed lymphoid cells.
TS28: spleen lymphoid follicle	Present	Regionally restricted	5c' , 5c	Expression is in tightly packed lymphoid cells in splenic nodules.

Structure	Level	Pattern	Image	Note
TS28: thymus medullary core	Present		5f' , 5f
TS28: thymus cortex	Weak		5f' , 5f

Structure	Level	Pattern	Image	Note
TS28: glomerular mesangium	Present		6a' , 6a
TS28: renal small blood vessel	Present		6a' , 6a

Structure	Level	Pattern	Image	Note
TS15: eye	Present	Regionally restricted	7a , 7a'	Expression is in the undifferentiated neuroepithelium.

Structure	Level	Pattern	Image	Note
TS21: neural retina	Present	Regionally restricted	7b	Expression is in undifferentiated layers in the distal region and is faint in the emergent ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		7c
TS15: neural tube	Present		7c
TS15: sensory ganglion	Present		7c
TS15: peripheral nervous system ganglion	Present	Regionally restricted	7c	Expression is in sympathetic ganglia.
TS15: dorsal root ganglion	Present	Regionally restricted	7c	Expression is in the periphery of the ganglia and in the area of ventral projections.

Structure	Level	Pattern	Image	Note
TS15: embryo ectoderm	Present	Single cells	7d' , 7d	Expression is in migrating melanoblasts.

Structure	Level	Pattern	Image	Note
TS21: skin	Present	Single cells	7e , 7e'	Expression is in neurons in the skin.
TS21: sensory ganglion	Present	Regionally restricted	7e' , 7e	Expression is in spinal rootlets and processes extending into premuscle masses in the limb.

Structure	Level	Pattern	Image	Note
TS15: chorionic plate	Present		8d' , 8a , 8d , 8a'	Expression is on blood vessels in the chorionic plate.
TS15: extraembryonic blood vessel	Present		8d' , 8a , 8d , 8a'	Expression is on blood vessels in the chorionic plate.
TS15: labyrinthine zone	Present	Single cells	8d , 8a' , 8d' , 8a
TS15: spongiotrophoblast	Present	Single cells	8d' , 8d , 8a , 8a'
TS15: trophectoderm	Present	Regionally restricted	8d' , 8a' , 8d , 8a	Expression is at the junction between the myometrium and layer of trophoblasts and is absent in the muscle cells.
TS15: trophoblast giant cell	Absent		8a , 8a' , 8d' , 8d

Structure	Level	Pattern	Image	Note
TS28: blood vessel	Present		8a , 8a'	Expression is in blood vessels throughout the placenta and myometrium.
TS28: myometrium	Absent		8a , 8a'	Expression is at the junction between the myometrium and layer of trophoblasts and is absent in the muscle cells.

Structure	Level	Pattern	Image	Note
TS15: chorionic plate	Present	Regionally restricted	8b' , 8b	Expression is on blood vessels in the chorionic plate.
TS15: extraembryonic blood vessel	Present	Regionally restricted	8b' , 8b	Expression is on blood vessels in the chorionic plate.
TS15: trophoblast giant cell	Absent		8b , 8b'

Structure	Level	Pattern	Image	Note
TS15: labyrinthine zone	Present	Single cells	8c' , 8c
TS15: trophoblast giant cell	Absent		8c' , 8c

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	8d , 8d'	Expression is in a region surrounding the blood vessel.

Structure	Level	Pattern	Image	Note
TS21: vitelline blood vessel	Present	Regionally restricted	9a' , 9a	Expression is in the smooth muscle.

Structure	Level	Pattern	Image	Note
TS21: vitelline blood vessel	Present	Regionally restricted	9b , 9b'	Expression is in the smooth muscle.
TS21: yolk sac mesenchyme	Present	Regionally restricted	9b , 9b'	Expression is in the blood islands.

Structure	Level	Pattern	Image	Note
TS21: yolk sac blood vessel	Present		9c , 9c'	Expression is in the blood islands. Expression is on the interior of the vessels.
TS21: yolk sac mesenchyme	Present		9c , 9c'	Expression is in the blood islands. Expression is on the interior of the vessels.

Structure	Level	Pattern	Image	Note
TS23: esophagus	Absent
TS23: trachea	Absent

Structure	Level	Pattern	Image	Note
TS28: aorta smooth muscle	Present
TS28: blood vessel	Present
TS28: blood vessel smooth muscle	Present
TS28: liver	Weak	Single cells