Gene Expression Data
Assay Details
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Assay
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Reference:
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J:47312
Hashimoto H, et al., Three-dimensional analysis of the developing pituitary gland in the mouse. Dev Dyn. 1998 May;212(1):157-66
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5910999
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Gene symbol:
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Lamb1
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Gene name:
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laminin B1
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Antibody:
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anti-laminin (AT-2404)
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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Fixative was 0.5% paraformaldehyde/15% picric acid. The authors state that the laminin antibody recognized both the a and b chains (Lama1 and Lamb1). Both genes have been annotated.
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Results
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Specimen
1:
embryonic day 10.5
(more  )
Specimen
1:
(close  )
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Genetic Background:
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ICR
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: Rathke's pouch |
Present |
Regionally restricted |
1
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Expression is at the basement membrane.
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Specimen
2:
embryonic day 11.5
(more )
Specimen
2:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Other - See Notes
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Specimen
7:
embryonic day 12.5
(more )
Specimen
7:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: pituitary gland |
Present |
Regionally restricted |
7
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Expression is at the basement membrane.
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Specimen
8:
embryonic day 13.5
(more )
Specimen
8:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: pituitary gland |
Present |
Regionally restricted |
8
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Expression is at the basement membrane. In the posterior wall are intercellular deposits of laminin, some of which reached the laminin sheet in the basement membrane.
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Specimen
13:
embryonic day 14.5
(more )
Specimen
13:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: pituitary gland |
Present |
Regionally restricted |
13
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Expression is at the basement membrane.
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Specimen
14:
embryonic day 15.5
(more )
Specimen
14:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pituitary gland |
Present |
Regionally restricted |
14
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Expression is at the basement membrane.
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Specimen
15:
embryonic day 15.5
(more )
Specimen
15:
(close )
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Genetic Background:
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ICR
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Age:
|
embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pituitary gland |
Present |
Regionally restricted |
15
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Expression is at the basement membrane.
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Specimen
19:
embryonic day 17.5
(more )
Specimen
19:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: pituitary gland |
Present |
Regionally restricted |
19
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Expression is at the basement membrane of the epithelial tissue and in the intercellular spaces between the epithelial cells.
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Specimen
20:
embryonic day 18.5
(more )
Specimen
20:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Other - See Notes
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: pituitary gland |
Present |
Regionally restricted |
20
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Expression is at the basement membrane.
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