Probe:	Pax1 probe29
Probe preparation:	Antisense RNA
Assay notes:	Control embryos were either heterozygotes or +/+. Embryos were post-fixed with 4% paraformaldhyde/0.2% glutaraldehyde.

Structure	Level	Pattern	Image	Note
TS14: paraxial mesenchyme	Present	Regionally restricted	3A control	Expression starts at the level of the 4th somite.
TS14: foregut	Present	Regionally restricted	3A control	Expression is in the pharynx.

Structure	Level	Pattern	Image	Note
TS14: paraxial mesenchyme	Present	Regionally restricted	3A -/-	Expression is the same as in wild-type embryos.
TS14: foregut	Present	Regionally restricted	3A -/-	Expression is in the pharynx. Expression is the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS15: somite	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 05	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 06	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 07	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 08	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 09	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 10	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 11	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 12	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 13	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 14	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 15	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 16	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 17	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 18	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 19	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 20	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 21	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 22	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 23	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 24	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 25	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 26	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 27	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 28	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 29	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: somite 30	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.
TS15: tail somite	Present	Regionally restricted	3B control	Expression is in all somites caudal to and including somite 4 and is highest in the more caudal somites.

Structure	Level	Pattern	Image	Note
TS15: somite	Weak	Regionally restricted	3B -/-	There is some expression in somites 4-7.
TS15: somite 05	Weak	Regionally restricted	3B -/-	There is some expression in somites 4-7.
TS15: somite 06	Weak	Regionally restricted	3B -/-	There is some expression in somites 4-7.
TS15: somite 07	Weak	Regionally restricted	3B -/-	There is some expression in somites 4-7.
TS15: paraxial mesenchyme	Present	Regionally restricted	3B -/-	Absent in caudal paraxial mesoderm.

Structure	Level	Pattern	Image	Note
TS15: trunk sclerotome	Strong		3C
TS15: trunk	Present	Regionally restricted	3C	Expression is as a band that crosses the midline ventral to the notochord.

Structure	Level	Pattern	Image	Note
TS15: somite	Weak	Regionally restricted	3D	Expression is ventral in this cervical somite.

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent