Gene Expression Data
Assay Details
Assay
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Reference:
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J:18543
Rashbass P, et al., Alterations in gene expression during mesoderm formation and axial patterning in Brachyury (T) embryos. Int J Dev Biol. 1994 Mar;38(1):35-44
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5910788
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Gene symbol:
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Evx1
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Gene name:
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even-skipped homeobox 1
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Probe:
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Evx1 probe9
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Probe preparation:
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Antisense RNA
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Assay notes:
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Control embryos were either heterozygotes or +/+. Embryos were post-fixed with 4% paraformaldhyde/0.2% glutaraldehyde.
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Results
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Specimen
1F control:
embryonic day 8.5
(more )
Note: 7 somite stage embryo.
Specimen
1F control:
(close )
Genetic Background:
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involves: BTBR/Pas
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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7 somite stage embryo.
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Specimen
1F -/-:
embryonic day 8.5; T/ T
(more )
Note: Approximately 7 somites.
Specimen
1F -/-:
(close )
Genetic Background:
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involves: BTBR/Pas
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Mutant Allele(s):
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T/T
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Approximately 7 somites.
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Structure |
Level |
Pattern |
Image |
Note |
TS13: embryo ectoderm |
Weak |
Regionally restricted |
1F -/-
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Expression in this mutant is the same as in wild-type embryos.
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TS13: embryo mesoderm |
Weak |
Regionally restricted |
1F -/-
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Expression in this mutant is the same as in wild-type embryos.
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TS13: neural ectoderm |
Weak |
Regionally restricted |
1F -/-
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Expression in this mutant is the same as in wild-type embryos.
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TS13: primitive streak |
Weak |
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1F -/-
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Expression in this mutant is the same as in wild-type embryos.
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TS13: embryo |
Weak |
Regionally restricted |
1F -/-
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Expression in this mutant is the same as in wild-type embryos.
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Specimen
1G control:
embryonic day 9.5
(more )
Specimen
1G control:
(close )
Genetic Background:
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involves: BTBR/Pas
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS15: tail |
Strong |
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1G control
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TS15: neural ectoderm |
Present |
Regionally restricted |
1G control
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Expression is in the caudal neurectoderm extending to the level of the most recently formed somite.
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Specimen
1G -/-:
embryonic day 9.5; T/ T
(more )
Specimen
1G -/-:
(close )
Genetic Background:
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involves: BTBR/Pas
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Mutant Allele(s):
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T/T
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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