Gene Expression Data
Assay Details
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Assay
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Reference:
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J:18543
Rashbass P, et al., Alterations in gene expression during mesoderm formation and axial patterning in Brachyury (T) embryos. Int J Dev Biol. 1994 Mar;38(1):35-44
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5910787
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Gene symbol:
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Msx1
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Gene name:
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msh homeobox 1
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Probe:
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Msx1 probe35
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Probe preparation:
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Antisense RNA
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Assay notes:
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Control embryos were either heterozygotes or +/+. Embryos were post-fixed with 4% paraformaldhyde/0.2% glutaraldehyde.
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Results
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Specimen
1C control:
embryonic day 8.5
(more  )
Specimen
1C control:
(close  )
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Genetic Background:
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involves: BTBR/Pas
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1C -/-:
embryonic day 8.5; T/ T
(more )
Note: 5 somite stage embryo.
Specimen
1C -/-:
(close )
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Genetic Background:
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involves: BTBR/Pas
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Mutant Allele(s):
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T/T
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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5 somite stage embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: amnion |
Present |
Regionally restricted |
1C -/-
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Expression in this tissue is the same as in wild-type embryos.
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| TS13: embryo ectoderm |
Present |
Regionally restricted |
1C -/-
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Expression in this tissue is the same as in wild-type embryos.
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| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
1C -/-
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Expression in this tissue is the same as in wild-type embryos.
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| TS13: neural fold |
Present |
Regionally restricted |
1C -/-
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Expression in this tissue is the same as in wild-type embryos.
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| TS13: neural plate |
Present |
Regionally restricted |
1C -/-
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Expression in this tissue is the same as in wild-type embryos.
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| TS13: primitive streak |
Present |
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1C -/-
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There is elevated expression in the caudal primitive streak in this mutant compared to wild-type.
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Specimen
1D control:
embryonic day 9.5
(more )
Specimen
1D control:
(close )
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Genetic Background:
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involves: BTBR/Pas
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1D -/-:
embryonic day 9.5; T/ T
(more )
Specimen
1D -/-:
(close )
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Genetic Background:
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involves: BTBR/Pas
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Mutant Allele(s):
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T/T
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1H:
embryonic day 9.5
(more )
Specimen
1H:
(close )
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Genetic Background:
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involves: BTBR/Pas
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
1I:
embryonic day 9.5; T/ T
(more )
Specimen
1I:
(close )
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Genetic Background:
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involves: BTBR/Pas
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Mutant Allele(s):
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T/T
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube |
Present |
Regionally restricted |
1I
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Expression the same as in wild-type embryos in this tissue.
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| TS15: neural crest |
Present |
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1I
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Expression is lateral to the dorsal neural tube in displaced neural crest cells.
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Analysis Tools