Gene Expression Data
Assay Details
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Assay
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Reference:
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J:18543
Rashbass P, et al., Alterations in gene expression during mesoderm formation and axial patterning in Brachyury (T) embryos. Int J Dev Biol. 1994 Mar;38(1):35-44
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5910780
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Gene symbol:
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Wnt5a
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Gene name:
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wingless-type MMTV integration site family, member 5A
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Probe:
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Wnt5a probe12
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Probe preparation:
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Antisense RNA
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Assay notes:
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Control embryos were either heterozygotes or +/+. Embryos were post-fixed with 4% paraformaldhyde/0.2% glutaraldehyde.
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Results
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Specimen
1A control:
embryonic day 8.5
(more  )
Note: 5-6 somites.
Specimen
1A control:
(close  )
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Genetic Background:
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involves: BTBR/Pas
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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5-6 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: embryo |
Present |
Regionally restricted |
1A control
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Expression is in tissue caudal to the last formed somite.
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| TS13: allantois |
Present |
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1A control
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Specimen
1A -/-:
embryonic day 8.5; T/ T
(more )
Note: 5-6 somites.
Specimen
1A -/-:
(close )
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Genetic Background:
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involves: BTBR/Pas
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Mutant Allele(s):
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T/T
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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5-6 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: embryo |
Absent |
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1A -/-
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There is no caudal expression in this mutant compared to wild-type.
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Specimen
not shown: control:
embryonic day 7.5
(more )
Specimen
not shown: control:
(close )
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Genetic Background:
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involves: BTBR/Pas
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
not shown: -/-:
embryonic day 7.5; T/ T
(more )
Specimen
not shown: -/-:
(close )
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Genetic Background:
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involves: BTBR/Pas
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Mutant Allele(s):
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T/T
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
not shown: 1-2 somites control:
embryonic day 8.0
(more )
Note: 1-2 somites.
Specimen
not shown: 1-2 somites control:
(close )
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Genetic Background:
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involves: BTBR/Pas
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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1-2 somites.
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Specimen
not shown: 1-2 somites -/-:
embryonic day 8.0; T/ T
(more )
Note: 1-2 somites.
Specimen
not shown: 1-2 somites -/-:
(close )
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Genetic Background:
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involves: BTBR/Pas
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Mutant Allele(s):
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T/T
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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1-2 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: allantois |
Present |
Regionally restricted |
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Expression in this mutant is the same as in wild-type embryos.
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| TS12: amnion |
Present |
Regionally restricted |
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Expression in this mutant is the same as in wild-type embryos.
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| TS12: embryo |
Present |
Regionally restricted |
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Expression in this mutant is the same as in wild-type embryos.
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| TS12: somite |
Present |
Regionally restricted |
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Expression in this mutant is the same as in wild-type embryos.
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Analysis Tools