Gene Expression Data
Assay Details
Assay
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Reference:
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J:89166
Oosterveen T, et al., Expression of retinaldehyde dehydrogenase II and sequential activation of 5' Hoxb genes in the mouse caudal hindbrain. Gene Expr Patterns. 2004 May;4(3):243-7
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5905597
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Gene symbol:
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Aldh1a2
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Gene name:
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aldehyde dehydrogenase family 1, subfamily A2
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Probe:
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Raldh2 probe12
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2B:
embryonic day 9.5
(more )
Specimen
2B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS15: paraxial mesenchyme |
Present |
Regionally restricted |
2B
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Strong expression in cervical mesenchyme with rather sharp anterior limit at level of boundary between somites 5 and 6.
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Specimen
2G:
embryonic day 10.5
(more )
Specimen
2G:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: paraxial mesenchyme |
Present |
Regionally restricted |
2G
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Sharp expression boundary at level between somites 5 and 6, marked by position of first dorsal root ganglion.
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Specimen
3A:
embryonic day 9.5
(more )
Specimen
3A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: paraxial mesenchyme |
Present |
Regionally restricted |
3A
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Expression in cervical mesenchyme with rostral boundary at level of boundary between somites 5 and 6.
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Specimen
3B:
embryonic day 11.5
(more )
Specimen
3B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: paraxial mesenchyme |
Present |
Regionally restricted |
3B
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Expression in ring-shaped structure giving position of 1st dorsal root ganglion at the level of somites 5/6.
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Specimen
3D:
embryonic day 11.5
(more )
Note: Double labeled: lighter blue - Raldh2 (Aldh1a2); darker blue - Hoxb6 (assay MGI:5905594).
Specimen
3D:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: lighter blue - Raldh2 (Aldh1a2); darker blue - Hoxb6 (assay MGI:5905594).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: paraxial mesenchyme |
Present |
Regionally restricted |
3D
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Anterior boundary of expression domain located more rostrally than a day earlier, and are more anterior than the cervical Raldh2 (Aldh1a2) domain.
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