Gene Expression Data
Assay Details
Assay
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Reference:
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J:86960
Darling DS, et al., Expression of Zfhep/deltaEF1 protein in palate, neural progenitors, and differentiated neurons. Gene Expr Patterns. 2003 Dec;3(6):709-17
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5905161
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Gene symbol:
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Zeb1
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Gene name:
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zinc finger E-box binding homeobox 1
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Antibody:
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anti-Zfhep
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Detection system:
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Secondary antibody coupled to rhodamine
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Results
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Specimen
2C/D:
embryonic day 14.5
(more )
Note: Double labeled: red - Zeb1; green - Myh3 (assay MGI:5905163).
Specimen
2C/D:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 14.5
|
Sex:
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Not Specified
|
Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Zeb1; green - Myh3 (assay MGI:5905163).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: forelimb muscle |
Present |
Regionally restricted |
2C
,
2D
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Expression in nuclei in myofibers.
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Specimen
2J/L:
embryonic day 13.5
(more )
Note: Double labeled: red - Zeb1; green - Myog (assay MGI:5905165).
Specimen
2J/L:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
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Double labeled: red - Zeb1; green - Myog (assay MGI:5905165).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: tongue muscle |
Present |
Regionally restricted |
2J
,
2L
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xpression in myoblasts.
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Specimen
2M/O:
embryonic day 13.5
(more )
Note: Double labeled: red - Zeb1; green - Myh3 (assay MGI:5905163).
Specimen
2M/O:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Zeb1; green - Myh3 (assay MGI:5905163).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: tongue muscle |
Present |
Regionally restricted |
2M
,
2O
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Expression in myotubes.
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Specimen
3D:
embryonic day 13.5
(more )
Note: Double labeled: red - Zeb1; green - Nefm (assay MGI:5905166).
Specimen
3D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Double labeled: red - Zeb1; green - Nefm (assay MGI:5905166).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: telencephalon ventricular layer |
Present |
Regionally restricted |
3D
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Expression in some but not all cells in ventricular zone. No overlap with neurofilament expression.
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Specimen
3E/G:
embryonic day 14.5
(more )
Note: Double labeled: red - Zeb1; green - Pcna (assay MGI:5905168).
Specimen
3E/G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
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Double labeled: red - Zeb1; green - Pcna (assay MGI:5905168).
|
Specimen
4B:
embryonic day 14.5
(more )
Note: Double labeled: red - Zeb1; green - Pcna (assay MGI:5905168).
Specimen
4B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Zeb1; green - Pcna (assay MGI:5905168).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: medulla oblongata |
Strong |
Regionally restricted |
4B
|
Strong expression in highly proliferative precerebellar neuroepithelium (PCN). Strong expression in anterior precerebellar extramural migratory stream (PEMS).
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