Antibody:	Anti-GR (PA1-511)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: palatal shelf epithelium	Present		3C , 3A	Expression was strongly nuclear with cytoplasmic staining also observed in epithelia. Expression was throughout the tissues with no apparent regionalization, and there appeared to be few cells without immunostaining. Immunostaining was significantly higher in epithelium compared to mesenchyme.
TS20: palatal shelf mesenchyme	Present		3C , 3A	Expression was strongly nuclear. Expression was throughout the tissues with no apparent regionalization, and there appeared to be few cells without immunostaining.

Structure	Level	Pattern	Image	Note
TS21: palatal shelf epithelium	Present			Expression localization and staining density was similar to that seen at E12.5.
TS21: palatal shelf mesenchyme	Present			Expression localization and staining density was similar to that seen at E12.5.

Structure	Level	Pattern	Image	Note
TS22: nasal epithelium	Present	Non-Uniform	3B	Expression differs between the upper epithelia distal to the palate and the cells adjacent to the palatal shelves.
TS22: palatal shelf mesenchyme	Present	Non-Uniform	3B , 3D	The expression appeared heterogeneous with both strongly and weakly expressing cells.

Structure	Level	Pattern	Image	Note
TS20: palatal shelf epithelium	Present	Uniform	4A
TS20: palatal shelf mesenchyme	Present	Uniform	4A

Structure	Level	Pattern	Image	Note
TS22: palatal shelf epithelium	Strong	Regionally restricted	4B	Strong expression was detected in the medial region in the fusion zone. Expression was both nuclear and cytoplasmic.
TS22: oral epithelium	Present	Regionally restricted	4B	Expression was higher in medial oral cells compared to the lateral oral region.

Structure	Level	Pattern	Image	Note
TS22: nasal epithelium	Present	Regionally restricted	5A	Expression in the upper nasal region had cytoplasmic localization.

Structure	Level	Pattern	Image	Note
TS22: nasal epithelium	Strong	Regionally restricted	5B	Expression in the nasal epithelium adjacent to the palate was strong in basal nuclei, and densitometric scores were higher for this region than the upper nasal cavity.

Structure	Level	Pattern	Image	Note
TS23: nasal epithelium	Strong	Regionally restricted	5C	Strong basal nuclear localization was observed. There was no differential staining in distal vs. proximal regions.

Structure	Level	Pattern	Image	Note
TS23: palatal shelf epithelium	Present	Regionally restricted	6A , 6C	Expression was detected in the medial epithelial cells in the central palatal region. Expression was in both nucleus and cytoplasm. Epithelial expression exceeded mesenchymal expression.
TS23: palatal shelf mesenchyme	Strong	Single cells	6C , 6A
TS23: oral epithelium	Present	Regionally restricted	6A , 6D , 6C	Medial oral epithelial cells (6C) had higher expression levels than the lateral cells (6D).

Structure	Level	Pattern	Image	Note
TS23: palatal shelf mesenchyme	Strong	Regionally restricted	6B	Expression was strong in the lateral palate in regions of chondrogenesis relative to other surrounding mesenchyme.