Antibody:	anti-MDA-9/syntenin
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: back skin	Absent		2A E12.5
TS20: skin	Absent		2A E12.5

Structure	Level	Pattern	Image	Note
TS22: back skin	Strong		2A E14.5	Expression was strong in most regions.
TS22: epidermis	Strong		2A E14.5	Expression was strong in most regions.
TS22: dermis	Trace		2A E14.5

Structure	Level	Pattern	Image	Note
TS24: epidermis	Present	Regionally restricted	2A E16.5	Expression is in the basal layer.
TS24: dermis	Present		2A E16.5

Structure	Level	Pattern	Image	Note
TS26: back skin	Trace		2A E18.5
TS26: dermis	Trace		2A E18.5
TS26: epidermis	Trace		2A E18.5

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Trace		2B E14.5

Structure	Level	Pattern	Image	Note
TS24: hair root sheath	Present		2B E16.5
TS24: connective tissue	Present	Regionally restricted	2B E16.5	Expression is in connective tissue surrounding the hair follicle.

Structure	Level	Pattern	Image	Note
TS26: hair inner root sheath	Present		2B E18.5
TS26: hair outer root sheath	Present		2B E18.5
TS26: connective tissue	Present	Regionally restricted	2B E18.5	Expression is in connective tissue surrounding the hair follicle.

Structure	Level	Pattern	Image	Note
TS20: vertebral cartilage condensation	Absent		3_E12.5

Structure	Level	Pattern	Image	Note
TS22: vertebral cartilage condensation	Strong		3_E14.5
TS22: connective tissue	Strong	Regionally restricted	3_E14.5	Expression is in the connective tissue surrounding the spinal column.

Structure	Level	Pattern	Image	Note
TS24: vertebral cartilage condensation	Strong		3_E16.5
TS24: connective tissue	Strong	Regionally restricted	3_E16.5	Expression is in the connective tissue surrounding the spinal column.

Structure	Level	Pattern	Image	Note
TS26: nucleus pulposus	Present		3_E18.5
TS26: vertebral cartilage condensation	Present		3_E18.5
TS26: connective tissue	Present	Regionally restricted	3_E18.5	Expression is in the connective tissue surrounding the spinal column.
TS26: annulus fibrosus	Absent		3_E18.5

Structure	Level	Pattern	Image	Note
TS20: atrium cardiac muscle	Strong		4_E12.5
TS20: ventricle cardiac muscle	Strong		4_E12.5

Structure	Level	Pattern	Image	Note
TS22: atrium cardiac muscle	Strong		4_E14.5
TS22: ventricle cardiac muscle	Strong		4_E14.5

Structure	Level	Pattern	Image	Note
TS24: atrium cardiac muscle	Strong		4_E16.5
TS24: ventricle cardiac muscle	Strong		4_E16.5

Structure	Level	Pattern	Image	Note
TS26: heart	Weak		4_E18.5

Structure	Level	Pattern	Image	Note
TS22: lung mesenchyme	Strong		5_E14.5
TS22: lung vascular element	Strong	Regionally restricted	5_E14.5	Expression is in endothelial cells.
TS22: lung epithelium	Absent		5_E14.5

Structure	Level	Pattern	Image	Note
TS24: lung mesenchyme	Strong		5_E16.5
TS24: lung vascular element	Strong	Regionally restricted	5_E16.5	Expression is in endothelial cells.
TS24: lung epithelium	Absent		5_E16.5

Structure	Level	Pattern	Image	Note
TS26: lung mesenchyme	Strong		5_E18.5
TS26: lung vascular element	Strong	Regionally restricted	5_E18.5	Expression is in endothelial cells.
TS26: lung epithelium	Absent		5_E18.5

Structure	Level	Pattern	Image	Note
TS20: liver	Weak		6_E12.5
TS20: liver vasculature	Weak	Regionally restricted	6_E12.5	Expression is in endothelial cells.

Structure	Level	Pattern	Image	Note
TS22: liver	Present		6_E14.5
TS22: liver vasculature	Present	Regionally restricted	6_E14.5	Expression is in endothelial cells.

Structure	Level	Pattern	Image	Note
TS24: liver	Present		6_E16.5
TS24: liver vasculature	Present	Regionally restricted	6_E16.5	Expression is in endothelial cells.

Structure	Level	Pattern	Image	Note
TS26: liver	Strong		6_E18.5
TS26: liver vasculature	Strong	Regionally restricted	6_E18.5	Expression is in endothelial cells.