Probe:	Gsh-1 probe4
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	The other probe (MGI:5882954) showed identical patterns at all time points.

Structure	Level	Pattern	Image	Note
TS19: metencephalon	Present	Regionally restricted	5A	Expressed in two stripes.
TS19: midbrain	Present	Regionally restricted	5A	Expressed in a broad band that caps the mesencephalic vesicle.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	5B/C	Discrete expression.
TS19: hindbrain	Present	Regionally restricted	5B/C	Expressed in myencephalon in the fourth ventricle.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	5D	Expressed as very well-defined stripes in the myencephalon.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	5E/F	Expressed as very well-defined stripes in the myencephalon.
TS19: metencephalon	Present	Regionally restricted	5E/F	Expressed as very well-defined stripes.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present		5G/H
TS19: prethalamus	Present		5G/H
TS19: telencephalon	Present	Regionally restricted	5G/H	Weaker expression in the ganglionic eminence.
TS19: diencephalon	Present	Regionally restricted	5G/H	Expressed around the optic recess.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	6A/B	Expressed in the mid-ventricular area, with very fine boundaries.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present	Regionally restricted	7A/B	Expressed as very well-defined stripes.
TS21: medulla oblongata	Present	Regionally restricted	7A/B	Expressed as very well-defined stripes.
TS21: midbrain	Present	Regionally restricted	7A/B	Expressed in a broad band that caps the mesencephalic vesicle.
TS21: hypothalamus	Present	Regionally restricted	7A/B	Expressed in the area immediately adjacent to the pituitary.
TS21: optic stalk	Strong		7A/B
TS21: medial ganglionic eminence	Present	Regionally restricted	7A/B	Weaker expression around the medial ganglionic eminence in the telencephalic vesicle.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present		7C/D
TS21: ganglionic eminence	Present		7C/D
TS21: hypothalamus	Present		7C/D
TS21: medulla oblongata	Present		7C/D
TS21: midbrain	Present		7C/D
TS21: optic stalk	Present		7C/D
TS21: thalamus	Present		7C/D

Structure	Level	Pattern	Image	Note
TS21: midbrain	Present	Regionally restricted	7E	Expressed in a broad band that caps the mesencephalic vesicle. The two continuous stripes from the neural tube can also be seen in the neural epithelial tissue surrounding the aqueduct.

Structure	Level	Pattern	Image	Note
TS21: diencephalon	Weak		7F
TS21: medulla oblongata	Weak		7F
TS21: pons	Present	Regionally restricted	7F	Expressed as very well-defined stripes.

Structure	Level	Pattern	Image	Note
TS21: ganglionic eminence	Present		7G/H
TS21: hypothalamus	Present		7G/H
TS21: thalamus	Present		7G/H
TS21: telencephalon	Present	Regionally restricted	7G/H	Expressed around the ganglionic eminence.