Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:76826
Zuniga A, et al., Mouse Twist is required for fibroblast growth factor-mediated epithelial-mesenchymal signalling and cell survival during limb morphogenesis. Mech Dev. 2002 Jun;114(1-2):51
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:5881819
|
|
Gene symbol:
|
Shh
|
|
Gene name:
|
sonic hedgehog
|
|
Probe:
|
Shh cDNA3
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
2A:
embryonic day 10.5
(more  )
Note: 33-35 somites
Specimen
2A:
(close  )
|
Genetic Background:
|
involves: C57BL/6J * CD-1
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
33-35 somites
|
Specimen
2B:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J * CD-1b
|
|
Mutant Allele(s):
|
Twist1tm1Bhr/Twist1tm1Bhr
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
33-35 somites
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: hindlimb bud |
Weak |
Regionally restricted |
2B
|
Expression is reduced in mutant hindlimb bud and expression domain is expanded more distal-anteriorly compared to wild -type.
|
Specimen
2C:
(close )
|
Genetic Background:
|
involves: 129S7/SvEvBrd * C57BL/6J * CD-1b
|
|
Mutant Allele(s):
|
Twist1tm1Bhr/Twist1tm1Bhr
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
33-35 somites
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: forelimb bud |
Trace |
|
2C
|
Authors report only low levels of expression in the mutant forelimb bud.
|
|
Analysis Tools