Probe:	Otx2 cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS8: epiblast	Weak		3a/a'

Structure	Level	Pattern	Image	Note
TS8: epiblast	Present		3b/b'

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Present	Widespread	3c/c'	Extended to the entire epiblast.

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Present	Regionally restricted	3h/h' , 3d/d'	Expression is confined to regions in the anterior third of the embryo.

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present	Regionally restricted	3e/e'	Restricted to the anterior embryonic ectoderm corresponding to presumptive fore- and midbrain.

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Present	Regionally restricted	3i/i'	Expressed noly in anterior regions, excluded from posterior and lateral ectoderm.

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Present	Regionally restricted	3j/j'	Expressed noly in anterior regions, excluded from posterior and lateral ectoderm.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	4d/d'	Expressed in noncortical basal telencephalic regions.
TS20: diencephalon	Present		4d/d'	With a pattern very similar to Otx1.
TS20: midbrain	Present		4d/d'	With a pattern very similar to Otx1.
TS20: nasal epithelium	Present		4d/d'

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	4e/e'	Expressed in noncortical basal telencephalic regions.
TS20: diencephalon	Present		4e/e'	With a pattern very similar to Otx1.
TS20: midbrain	Present		4e/e'	With a pattern very similar to Otx1.
TS20: nasal epithelium	Present		4e/e'
TS20: neurohypophysis	Present		4e/e'
TS20: choroid plexus	Present	Regionally restricted	4e/e'	Expressed in outer ventriculi.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	4f/f'	Expressed in noncortical basal telencephalic regions.
TS20: diencephalon	Present		4f/f'	With a pattern very similar to Otx1.
TS20: midbrain	Present		4f/f'	With a pattern very similar to Otx1.
TS20: hindbrain	Present		4f/f'
TS20: nasal epithelium	Present		4f/f'

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Very strong	Regionally restricted	5d/d'	Expressed in septal regions contiguous to diencephalon and in the germinal layer of anterior basimedial regions.
TS20: diencephalon	Present		5d/d'	With a pattern very similar to Otx1.
TS20: midbrain	Present		5d/d'	With a pattern very similar to Otx1.
TS20: nasal epithelium	Present		5d/d'
TS20: neurohypophysis	Present		5d/d'
TS20: choroid plexus	Present	Regionally restricted	5d/d'	Expressed in outer ventriculi.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present		5e/e'	With a pattern very similar to Otx1.
TS20: midbrain	Present		5e/e'	With a pattern very similar to Otx1.
TS20: ear	Present		5e/e'
TS20: eye	Present		5e/e'
TS20: nasal epithelium	Present		5e/e'
TS20: neurohypophysis	Present		5e/e'
TS20: choroid plexus	Present	Regionally restricted	5e/e'	Expressed in outer ventriculi.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present		5f/f'	With a pattern very similar to Otx1.
TS20: midbrain	Present		5f/f'	With a pattern very similar to Otx1.

Structure	Level	Pattern	Image	Note
TS20: diencephalon lamina terminalis	Present		6c
TS20: diencephalon	Present	Regionally restricted	6c	Expressed in the dorsal region. Expressed in germinal layers and boundary regions.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	6h	Expressed in ependymal layers of epithalamus and dorsal thalamus and in orthogonal layers of cells immediately above and below the sulcus diencephalicus medius.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	6i	Expressed in ependymal layers of epithalamus and dorsal thalamus and in orthogonal layers of cells immediately above and below the sulcus diencephalicus medius.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	6l	Expressed in basal telencephalic regions, more superficial.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	6m	Expressed in basal telencephalic regions, more superficial.

Structure	Level	Pattern	Image	Note
TS20: nasal epithelium	Present	Regionally restricted	7b/b'	Not expressed in the lateral wall of respiratory pits.
TS20: olfactory epithelium	Present		7b/b'

Structure	Level	Pattern	Image	Note
TS16: optic stalk	Present		7f

Structure	Level	Pattern	Image	Note
TS20: eye	Present	Regionally restricted	7g	Expressed in the sclera.
TS20: pigmented retinal epithelium	Present		7g
TS20: meninges	Present	Regionally restricted	7g	Expressed in leptomeninges intimately surrounding optic nerve.

Structure	Level	Pattern	Image	Note
TS25: neural retina	Present		7h
TS25: pigmented retinal epithelium	Present		7h

Structure	Level	Pattern	Image	Note
TS16: otocyst	Present		7k/k'

Structure	Level	Pattern	Image	Note
TS20: cochlear duct epithelium	Present		7l/l'
TS20: saccule epithelium	Present		7l/l'

Structure	Level	Pattern	Image	Note
TS7: epiblast	Weak

Structure	Level	Pattern	Image	Note
TS23: brain	Present			Expression remains essentially the same up to E15 embryos and progressively declines later on.