Gene Expression Data
Assay Details
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Assay
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Reference:
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J:142456
Gustin SE, et al., Testis development, fertility, and survival in Ethanolamine kinase 2-deficient mice. Endocrinology. 2008 Dec;149(12):6176-86
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5827662
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Gene symbol:
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Etnk2
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Gene name:
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ethanolamine kinase 2
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Reporter:
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GFP
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Antibody:
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anti-GFP (Rockland)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
3B 10.5:
(close  )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Etnk2tm1Sinc/Etnk2tm1Sinc
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Age:
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embryonic day 10.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: gonad primordium |
Present |
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3B 10.5
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The authors state that fluorescence was not detected in any other tissues.
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Specimen
3B 11.5:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Etnk2tm1Sinc/Etnk2tm1Sinc
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Age:
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embryonic day 11.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: gonad |
Strong |
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3B 11.5
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The authors state that fluorescence was not detected in any other tissues.
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Specimen
3B 12.5:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Etnk2tm1Sinc/Etnk2tm1Sinc
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Age:
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embryonic day 12.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: primary sex cord |
Present |
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3B 12.5
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The authors state that fluorescence was not detected in any other tissues.
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Specimen
3B 13.5:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Etnk2tm1Sinc/Etnk2tm1Sinc
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Age:
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embryonic day 13.5
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Sex:
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Male
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: primary sex cord |
Present |
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3B 13.5
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The authors state that fluorescence was not detected in any other tissues.
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Specimen
not shown: female:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Etnk2tm1Sinc/Etnk2tm1Sinc
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Age:
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embryonic day 10.5
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
C top:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Etnk2tm1Sinc/Etnk2tm1Sinc
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Age:
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embryonic day 13.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Etnk2; red - Wt1 (assay MGI:5827713).
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Specimen
C middle:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Etnk2tm1Sinc/Etnk2tm1Sinc
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Age:
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embryonic day 13.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Etnk2; red - Cyp11a1 (assay MGI:5827712).
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Specimen
C bottom:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Etnk2tm1Sinc/Etnk2tm1Sinc
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Age:
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embryonic day 13.5
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Etnk2; red - Ddx4 (assay MGI:5827715).
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Specimen
C Adult:
(close )
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Genetic Background:
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involves: C57BL/6
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Mutant Allele(s):
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Etnk2tm1Sinc/Etnk2tm1Sinc
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Etnk2; red - Ddx4 (assay MGI:5827715).
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)
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