Etnk2 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:142456 Gustin SE, et al., Testis development, fertility, and survival in Ethanolamine kinase 2-deficient mice. Endocrinology. 2008 Dec;149(12):6176-86
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:5827662
Gene symbol:	Etnk2
Gene name:	ethanolamine kinase 2

Reporter:	GFP
Antibody:	anti-GFP (Rockland)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

Specimen 3B 10.5: embryonic day 10.5; Etnk2^tm1Sinc/Etnk2^tm1Sinc (more

)

Structure	Level	Pattern	Image	Note
TS17: gonad primordium	Present		3B 10.5	The authors state that fluorescence was not detected in any other tissues.

Specimen 3B 11.5: embryonic day 11.5; Etnk2^tm1Sinc/Etnk2^tm1Sinc (more

)

Structure	Level	Pattern	Image	Note
TS19: gonad	Strong		3B 11.5	The authors state that fluorescence was not detected in any other tissues.

Specimen 3B 12.5: embryonic day 12.5; Etnk2^tm1Sinc/Etnk2^tm1Sinc (more

)

Structure	Level	Pattern	Image	Note
TS20: primary sex cord	Present		3B 12.5	The authors state that fluorescence was not detected in any other tissues.

Specimen 3B 13.5: embryonic day 13.5; Etnk2^tm1Sinc/Etnk2^tm1Sinc (more

)

Structure	Level	Pattern	Image	Note
TS21: primary sex cord	Present		3B 13.5	The authors state that fluorescence was not detected in any other tissues.

Specimen not shown: female: embryonic day 10.5; Etnk2^tm1Sinc/Etnk2^tm1Sinc (more

)

Structure	Level	Pattern	Image	Note
TS17: gonad primordium	Present

Specimen C top: embryonic day 13.5; Etnk2^tm1Sinc/Etnk2^tm1Sinc (more

)
Note: Double labeled: green - Etnk2; red - Wt1 (assay MGI:5827713).

Structure	Cell Type	Level	Image
TS21: interstitium of the testis		Present	3C 13.5 EGFP top , 3C 13.5 Merge top
TS21: Sertoli cell	Sertoli cell	Present	3C 13.5 EGFP top , 3C 13.5 Merge top
TS21: germ cell of testis		Absent	3C 13.5 EGFP top , 3C 13.5 Merge top

Specimen C middle: embryonic day 13.5; Etnk2^tm1Sinc/Etnk2^tm1Sinc (more

)
Note: Double labeled: green - Etnk2; red - Cyp11a1 (assay MGI:5827712).

Structure	Cell Type	Level	Image
TS21: interstitium of the testis		Present	3C 13.5 EGFP middle , 3C 13.5 Merge middle
TS21: Sertoli cell	Sertoli cell	Present	3C 13.5 Merge middle , 3C 13.5 EGFP middle
TS21: germ cell of testis		Absent	3C 13.5 EGFP middle , 3C 13.5 Merge middle

Specimen C bottom: embryonic day 13.5; Etnk2^tm1Sinc/Etnk2^tm1Sinc (more

)
Note: Double labeled: green - Etnk2; red - Ddx4 (assay MGI:5827715).

Structure	Cell Type	Level	Image
TS21: interstitium of the testis		Present	3C 13.5 Merge bottom , 3C 13.5 EGFP bottom
TS21: Sertoli cell	Sertoli cell	Present	3C 13.5 EGFP bottom , 3C 13.5 Merge bottom
TS21: germ cell of testis		Absent	3C 13.5 EGFP bottom , 3C 13.5 Merge bottom

Specimen C Adult: postnatal adult; Etnk2^tm1Sinc/Etnk2^tm1Sinc (more

)
Note: Double labeled: green - Etnk2; red - Ddx4 (assay MGI:5827715).

Structure	Cell Type	Level	Image
TS28: interstitium of the testis		Strong	3C Adult EGFP , 3C Adult Merge
TS28: seminiferous tubule		Present	3C Adult EGFP , 3C Adult Merge
TS28: Sertoli cell	Sertoli cell	Present	3C Adult EGFP , 3C Adult Merge

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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