Etnk2^tm1Sinc
Targeted Allele Detail

Summary

• Symbol: Etnk2^tm1Sinc
• Name: ethanolamine kinase 2; targeted mutation 1, Andrew Sinclair
• MGI ID: MGI:3823016
• Synonyms: Eki2^-, Eki^eGFP
• Gene: Etnk2 Location: Chr1:133291310-133308074 bp, + strand Genetic Position: Chr1, 57.91 cM
• Alliance: Etnk2^tm1Sinc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:142456
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene locus was inactivated by inserting the enhanced green fluorescence protein (GFP) coding sequence in-frame into the ATG-start site of Eki2, replacing part of exon 1 and exon 2. A loxP-flanked neo selection cassette was included downstream of the GFP cassette and was subsequently removed by crossing mice with CMV-cre transgenic mice. Gene inactivation was confirmed by quantitative PCR of testis RNA. GFP expression was observed in the testis of E13.5 embryos and adult males. (J:142456)

Expression

In Mice Carrying this Mutation:

27 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Etnk2 Mutation: 23 strains or lines available

References

• Original: J:142456 Gustin SE, et al., Testis development, fertility, and survival in Ethanolamine kinase 2-deficient mice. Endocrinology. 2008 Dec;149(12):6176-86
• All: 1 reference(s)