Prrx1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:72551 Chesterman ES, et al., Investigation of Prx1 protein expression provides evidence for conservation of cardiac-specific posttranscriptional regulation in vertebrates. Dev Dyn. 2001 Nov;222(3):459-70
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5824839
Gene symbol:	Prrx1
Gene name:	paired related homeobox 1

Antibody:	Prrx1 antibody
Assay notes:	Fixative was: 85% ethanol, 3.7% formalin, 5% acetic acid.

Results

3A ©

Specimen 3A: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mesenchyme	Present	Graded	3A	Highest expression is distal.
TS17: 2nd branchial arch mesenchyme	Present	Graded	3A	Highest expression is distal.
TS17: 1st branchial arch ectoderm	Absent		3A
TS17: 2nd branchial arch ectoderm	Absent		3A

3B ©

Specimen 3B: embryonic day 10.5; Prrx1^tm1Tex/Prrx1^tm1Tex (more

)

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Absent		3B

3C ©

Specimen 3C: embryonic day 10.5; Prrx1^tm1Tex/Prrx1^tm1Tex, Prrx2^tm1Mjk/Prrx2^tm1Mjk (more

)

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Absent		3C
TS17: 2nd branchial arch	Absent		3C

3D ©

Specimen 3D: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Graded	3D	Highest expression is distal.
TS17: forelimb bud ectoderm	Absent		3D

3E ©

Specimen 3E: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: foot mesenchyme	Present	Regionally restricted	3E	Expression is in the mesenchyme surrounding the phalanges.

3F ©

Specimen 3F: embryonic day 16.5; Prrx1^tm1Tex/Prrx1^tm1Tex (more

)

Structure	Level	Pattern	Image	Note
TS24: foot mesenchyme	Absent		3F

3G ©

Specimen 3G: embryonic day 16.5; Prrx1^tm1Tex/Prrx1^tm1Tex, Prrx2^tm1Mjk/Prrx2^tm1Mjk (more

)

Structure	Level	Pattern	Image	Note
TS24: foot mesenchyme	Absent		3G

4A ©

Specimen 4A: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: hindlimb mesenchyme	Present	Regionally restricted	4A	Expression is at the periphery of the developing limb away from bone-forming condensations.

4B ©

Specimen 4B: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: inner ear mesenchyme	Present	Regionally restricted	4B	Expression is in proximity to the pharyngotympanic tube in the undifferentiated mesenchyme that will form structures of the inner ear.

4C ©

Specimen 4C: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: nasal mesenchyme	Present	Regionally restricted	4C	Expression is in the mesenchyme surrounding the cartilage of the nasal septum.

4D ©

Specimen 4D: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: face mesenchyme	Strong	Regionally restricted	4D	Expression is in the mesenchyme immediately underlying the epithelium of the whisker follicles.

4E ©

Specimen 4E: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: humerus cartilage condensation	Present	Regionally restricted	4E	Expression is in mesenchymal cells at the periphery.
TS22: radius cartilage condensation	Present	Regionally restricted	4E	Expression is in mesenchymal cells at the periphery.
TS22: hand mesenchyme	Present	Regionally restricted	4E	Expression is in mesenchyme surrounding the phalanges.

4G ©

Specimen 4G: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: palate mesenchyme	Present		4G
TS24: tongue mesenchyme	Present		4G

4H ©

Specimen 4H: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: mandible	Present	Regionally restricted	4H	Expression is in the mesenchyme of the mandible surrounding Meckels cartilage.

4I ©

Specimen 4I: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: lower jaw molar mesenchyme	Present		4I

4J ©

Specimen 4J: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: foot mesenchyme	Present	Regionally restricted	4J	Expression is surrounding the forming digits.
TS24: hindlimb phalanx	Present	Regionally restricted	4J	Expression is in the periosteum.

5A ©

Specimen 5A: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present		5A

5B ©

Specimen 5B: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: atrioventricular endocardial cushion	Absent		5B

5D ©

Specimen 5D: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: forelimb	Present		5D

5E ©

Specimen 5E: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: atrioventricular endocardial cushion	Absent		5E

5G ©

Specimen 5G: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: hindlimb digit	Present		5G

5H ©

Specimen 5H: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: atrioventricular valve	Absent		5H

Specimen not shown 10.5: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Present	Graded		Highest expression is distal.

Specimen not shown 12.5: embryonic day 12.5 (more

)

Structure	Level	Pattern	Note
TS20: mandibular process mesenchyme	Present	Regionally restricted	Expression is in the undifferentiating mesenchyme.
TS20: maxillary process mesenchyme	Present	Regionally restricted	Expression is in the undifferentiating mesenchyme.
TS20: forelimb	Present

Specimen not shown Prrx1-/-;Prrx2-/- E12.5: embryonic day 12.5; Prrx1^tm1Tex/Prrx1^tm1Tex, Prrx2^tm1Mjk/Prrx2^tm1Mjk (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb	Absent
TS20: hindlimb mesenchyme	Absent
TS20: inner ear mesenchyme	Absent
TS20: mandibular process mesenchyme	Absent
TS20: maxillary process mesenchyme	Absent

Specimen not shown E14.5: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: mandible	Strong	Regionally restricted		Expression is adjacent to the developing tooth buds.
TS22: head	Present	Regionally restricted		Expression is in the undifferentiating tissue surrounding the first branchial cleft and the cartilage primordium of the stapes.

Specimen not shown Prrx1-/-;/Prrx2-/- E14.5: embryonic day 14.5; Prrx1^tm1Tex/Prrx1^tm1Tex, Prrx2^tm1Mjk/Prrx2^tm1Mjk (more

)

Structure	Level	Pattern	Image	Note
TS22: hand mesenchyme	Absent
TS22: humerus cartilage condensation	Absent
TS22: nasal mesenchyme	Absent
TS22: radius cartilage condensation	Absent

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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