Probe:	Dach2 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Absent		4A
TS13: future prosencephalon	Absent		4A
TS13: future hindbrain	Present	Regionally restricted	4A	Expression within hindbrain, anterior to preotic sulcus.
TS13: somite	Absent		4A	No expression in developing somites.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	4B	Expression within ventral forebrain.
TS15: branchial arch	Present		4B
TS15: future hindbrain	Present		4B
TS15: head mesenchyme	Present		4B
TS15: neural tube	Present		4B
TS15: otic pit	Present		4B
TS15: somite	Present	Regionally restricted	4B	Authors report expression in trunk mesoderm lateral to the neural tube and in the interlimb bud region in a repeated pattern suggesting expression in somites.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present		4C
TS17: telencephalon	Present		4C
TS17: midbrain	Present	Regionally restricted	4C	Expression in dorsal mesencephalon.
TS17: neural tube	Present	Regionally restricted	4C	Expression in dorsal neural tube, with highest expression near the hindbrain.
TS17: lens pit	Present	Regionally restricted	4C	Authors report a small ring of ocular staining suggesting expression in the lens pit.
TS17: inner ear	Present	Regionally restricted	4C	Expression in the otic endolymphatic duct.
TS17: branchial arch	Present		4C
TS17: dorsal root ganglion	Present		4C
TS17: head mesenchyme	Present		4C
TS17: somite	Present	Regionally restricted	4C	Expression in a repeated pattern in trunk mesoderm lateral to the neural tube and in the interlimb bud region suggesting expression in somite derivatives.
TS17: forelimb bud	Present	Regionally restricted	4C	Expression in anterior and posterior margins and presumptive handplate of limb bud.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present		4D
TS19: telencephalon	Present		4D
TS19: midbrain	Present	Regionally restricted	4D	Expression in dorsal mesencephalon.
TS19: neural tube	Present	Regionally restricted	4D	Expression in dorsal neural tube, with highest expression near the hindbrain.
TS19: inner ear	Present	Regionally restricted	4D	Expression in the otic endolymphatic duct.
TS19: forelimb bud	Present	Regionally restricted	4D	Expression in anterior and posterior margins.
TS19: handplate	Present	Regionally restricted	4D
TS19: somite	Present	Regionally restricted	4D	Expression in a repeated pattern in trunk mesoderm lateral to the neural tube and in the interlimb bud region suggesting expression in somite derivatives.
TS19: cranial ganglion	Present		4D
TS19: dorsal root ganglion	Present		4D
TS19: eye mesenchyme	Present	Regionally restricted	4D	Expression in mesenchyme surrounding eye.
TS19: branchial arch	Present	Regionally restricted	4D	Expression in branchial arches, with strong expression at the cranial sinus.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present		4E
TS20: telencephalon	Present		4E
TS20: midbrain	Present	Regionally restricted	4E	Expression in dorsal mesencephalon.
TS20: spinal cord	Present	Regionally restricted	4E	Expression in dorsal neural tube (highest expression near the hindbrain.)
TS20: retina	Strong	Regionally restricted	4E	Expression is more pronounced in the retina.
TS20: neural retina	Weak	Regionally restricted	4E	Low levels of expression in the peripheral neuroretina.
TS20: inner ear	Present	Regionally restricted	4E	Expression in the otic endolymphatic duct.
TS20: forelimb	Present	Regionally restricted	4E	Expression in the limb has become more uniform and higher in the distal edges (margins) of the limb plate.
TS20: somite	Present	Regionally restricted	4E	Expression in a repeated pattern in trunk mesoderm lateral to the neural tube and in the interlimb bud region suggesting expression in somite derivatives.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	4F	Striped expression in telencephalic vesicles.
TS20: midbrain	Present	Regionally restricted	4F	Expression in dorsal mesencephalon.
TS20: eye	Present		4F
TS20: hindbrain	Present		4F

Structure	Level	Pattern	Image	Note
TS22: genital tubercle	Present		4G
TS22: main olfactory bulb	Present		4G
TS22: vibrissa	Present		4G
TS22: hand	Present	Regionally restricted	4G	Expression in hand plate and lateral edges of digits.
TS22: mammary gland primordium	Present	Regionally restricted	4G	Expression in mammary gland primordia on the abdomen and associated with each limb.
TS22: naris	Present		4G	Expression in nasal opening.

Structure	Level	Pattern	Image	Note
TS22: hand	Present	Regionally restricted	4H	Expression in hand plate and lateral edges of digits.
TS22: naris	Present		4H	Expression in nasal opening and vibrissae.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	5A	Expression within forebrain neurectoderm flanked the optic vesicle with rostral and caudal restrictions.
TS15: future midbrain	Present	Regionally restricted	5A	Expression in dorsal and ventral domains in hindbrain.
TS15: eye mesenchyme	Present	Regionally restricted	5A	Expression in head mesoderm associated with the developing eye.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	5B	Expression in the dorsal and ventral neural tube.
TS15: branchial arch	Present		5B
TS15: dorsal root ganglion	Present		5B
TS15: head mesenchyme	Present		5B
TS15: mesenchyme derived from lateral plate	Present		5B
TS15: dermomyotome	Ambiguous		5B	In sections anterior to the forelimb, authors state expression in dermomyotome was not as readily detectable.
TS15: limb mesenchyme	Present	Regionally restricted	5B	Expression within a dorsal domain within the limb bud.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	5C	Expression within neuroectoderm distal to the developing optic vesicle is stronger than weak expression near the vesicle rim.
TS15: optic vesicle	Absent		5C	No expression within the optic vesicle or in the overying lens placode ectoderm.
TS15: eye mesenchyme	Present	Regionally restricted	5C	Expression in mesoderm adjacent to optic vesicle.
TS15: branchial arch	Present		5C
TS15: head mesenchyme	Present		5C

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	5D	In the trunk, expression in dorsal neural tube.
TS17: dorsal root ganglion	Present	Regionally restricted	5D
TS17: limb mesenchyme	Present	Regionally restricted	5D
TS17: mesenchyme derived from lateral plate	Present	Regionally restricted	5D

Structure	Level	Pattern	Image	Note
TS17: lens pit	Present	Regionally restricted	5E	Expression in the lens epithelium.
TS17: eye mesenchyme	Present	Regionally restricted	5E	Expression in the mesenchyme surrounding retina.
TS17: retina	Weak	Regionally restricted	5E	Low levels of expression in the peripheral neuroretina.
TS17: head mesenchyme	Present	Regionally restricted	5E	Expression in head mesoderm associated with the developing eye.
TS17: branchial arch	Present		5E

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present		5F
TS17: mesenchyme derived from lateral plate	Present		5F

Structure	Level	Pattern	Image	Note
TS15: embryo	Present			Authors report no abrogation of expression in the mutant compared to wild type embryos.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present			Authors report no abrogation of expression in the mutant compared to wild type embryos.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	6A	Expression in forebrain at level of optic vesicle.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	6B	Expression in forebrain at level of optic vesicle. Authors report no detectable differences in the mutant compared to wild type embryos.

Structure	Level	Pattern	Image	Note
TS15: dermomyotome	Ambiguous		6C	In sections anterior to the forelimb, authors state expression in dermomyotome was not as readily detectable.
TS15: trunk mesenchyme	Present	Regionally restricted	6C	Expression in cells located along the lateral portion of the trunk.
TS15: limb bud	Present	Regionally restricted	6C	Expression in within a dorsal domain within the limb bud.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		6D	Authors report no gross differences in expression in the dorsal neural tube in the mutant compared to wild type embryos.

Structure	Level	Pattern	Image	Note
TS15: dermomyotome	Present		6E	In sections at forelimb level, expression within the dermomyotome.
TS15: trunk mesenchyme	Present	Regionally restricted	6E	Expression in cells located along the lateral portion of the trunk.
TS15: limb bud	Present	Regionally restricted	6E	Expression in within a dorsal domain within the limb bud.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		6F	Authors report no gross differences in expression in the dermomyotome in the mutant compared to wild type embryos.