Wsb2 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:121557 Sarraj MA, et al., Expression of Wsb2 in the developing and adult mouse testis. Reproduction. 2007 Apr;133(4):753-761
Assay type:	RT-PCR
MGI Accession ID:	MGI:5817913
Gene symbol:	Wsb2
Gene name:	WD repeat and SOCS box-containing 2

Probe:	Wsb2-pA, Wsb2-pB
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Relative levels of expression are shown normalized to 18S expression.

Results

Image: 6A

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
12.5 M	E12.5	TS20: testis	Present	Not Specified; total RNA	CD-1		Male
12.5 F	E12.5	TS20: ovary	Present	Not Specified; total RNA	CD-1		Female
14.5 M	E14.5	TS22: testis	Present (a)	Not Specified; total RNA	CD-1		Male
14.5 F	E14.5	TS22: ovary	Present (a)	Not Specified; total RNA	CD-1		Female
16.5 M	E16.5	TS24: testis	Present (b)	Not Specified; total RNA	CD-1		Male
16.5 F	E16.5	TS24: ovary	Present (c)	Not Specified; total RNA	CD-1		Female
D0M	P0	TS27: testis	Present (d)	Not Specified; total RNA	CD-1		Male
D0F	P0	TS27: ovary	Present	Not Specified; total RNA	CD-1		Female

Notes:
(a) There was a 3-fold increase in expression between E12.5 and E14.5.
(b) There was a 6-fold increase in expression between E12.5 and E16.5.
(c) There was a 2-fold increase in expression between E12.5 and E16.5.
(d) There was a 6.6-fold increase in expression between E12.5 and the day of birth. Expression was 7-fold higher in newborn testis compared to newborn ovaries.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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