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Gene Expression Data
Assay Details
Assay
Reference: J:214682 Wei Z, et al., Rufy3, a protein specifically expressed in neurons, interacts with actin-bundling protein Fascin to control the growth of axons. J Neurochem. 2014 Sep;130(5):678-92
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5816577
Gene symbol: Rufy3
Gene name: RUN and FYVE domain containing 3
Antibody: Anti-Rufy3
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 1c: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: telencephalon Present Regionally restricted 1c Expression was detected in the cortex, where it appeared restricted to the upper layers but was not found in the ventricular zone.
TS19: telencephalon ventricular layer Absent 1c
Specimen 1d top: embryonic day 18.5 (more )
Note: Double labeled: red - Rufy3; green - Tubb3 (assay MGI:5816578).
Structure Level Pattern Image Note
TS26: cerebral cortex Present Regionally restricted 1d Rufy3 top , 1d Merge top Expression was coexpressed with Tubb3 in the upper layers of the cortex.
TS26: brain Present Regionally restricted 1d Rufy3 top , 1d Merge top Expression was detected in brain regions where neurons were abundant.
Specimen 1d bottom: embryonic day 18.5 (more )
Note: Double labeled: red - Rufy3; green - Nes (assay MGI:5816579).
Structure Level Pattern Image Note
TS26: cerebral cortex Present Regionally restricted 1d Rufy3 bottom , 1d Merge bottom Expression was detected in the upper layers of the cortex. There was not co-expression with Nestin.
TS26: brain Present Regionally restricted 1d Rufy3 bottom , 1d Merge bottom Expression was detected in brain regions where neurons were abundant.
Specimen 1e top: embryonic day 18.5 (more )
Note: Double labeled: red - Rufy3; green - Nes (assay MGI:5816579).
Structure Level Pattern Image Note
TS26: cerebral cortex intermediate zone Present 1e Rufy3 top , 1e Merge top Expression was different from Nestin.
TS26: cortical plate Present 1e Rufy3 top , 1e Merge top Expression was different from Nestin.
Specimen 1e middle: embryonic day 18.5 (more )
Note: Double labeled: red - Rufy3; green - Tubb3 (assay MGI:5816578).
Structure Level Pattern Image Note
TS26: cerebral cortex intermediate zone Present 1e Merge middle , 1e Rufy3 middle Expression was identical to Tubb3.
TS26: cortical plate Present 1e Merge middle , 1e Rufy3 middle Expression was identical to Tubb3.
Specimen 1e bottom: embryonic day 18.5 (more )
Note: Double labeled: red - Rufy3; green - Map2 (assay MGI:5816580).
Structure Level Pattern Image Note
TS26: cerebral cortex intermediate zone Present 1e Merge bottom , 1e Rufy3 bottom Expression was present in dendrites containing Map2.
TS26: cortical plate Present 1e Merge bottom , 1e Rufy3 bottom Expression was present in dendrites containing Map2.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory