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Gene Expression Data
Assay Details
Assay
Reference: J:226028 Lewandowski JP, et al., Spatiotemporal regulation of GLI target genes in the mammalian limb bud. Dev Biol. 2015 Oct 1;406(1):92-103
Assay type: RT-PCR
MGI Accession ID: MGI:5812452
Gene symbol: Rasgef1b
Gene name: RasGEF domain family, member 1B
Probe: Rasgef1b-pA, Rasgef1b-pB
Assay notes: qRT-PCR was used to obtain these results. Single forelimb pairs were used for each sample; 4 embryos were used per genotype. Data was normalized to a single pair of wild-type forelimbs using Gapdh expression across all samples.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
Wild-type E10.5 (a) TS17: forelimb bud Present 0.3 µg involves: 129S1/Sv * 129X1/SvJ Not Specified
Shh-/-;Gli3-/- E10.5 (a) TS17: forelimb bud Present (b) 0.3 µg involves: 129S1/Sv * 129X1/SvJ Gli3Xt-J/Gli3Xt-J, Shhtm1Amc/Shhtm1AmcNot Specified
Notes:
(a) 34-36 somite stage
(b) Expression levels are unchanged between mutant and wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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