Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:81021
Schmuth M, et al., Role of Peroxisome Proliferator-Activated Receptor alpha in Epidermal Development in Utero. J Invest Dermatol. 2002 Dec;119(6):1298-303
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5811573
|
|
Gene symbol:
|
Loricrin
|
|
Gene name:
|
loricrin cornified envelope precursor protein
|
|
Antibody:
|
anti-Loricrin
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
Assay notes:
|
Fixative was 4% formaldehyde.
|
|
|
Results
|
Specimen
4 +/+ 18.5:
embryonic day 18.5
(more  )
Specimen
4 +/+ 18.5:
(close  )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6N
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Formaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: epidermis |
Present |
Regionally restricted |
4_+/+ 18.5d
|
Expression is in the cornified envelope of the ventral skin.
|
Specimen
4 -/- 18.5:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6N
|
|
Mutant Allele(s):
|
Pparatm1Gonz/Pparatm1Gonz
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Formaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: epidermis |
Weak |
Regionally restricted |
4_-/- 18.5d
|
Expression is in the cornified envelope of the ventral skin.
|
Specimen
4 +/+ birth:
postnatal newborn
(more )
Specimen
4 +/+ birth:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6N
|
|
Age:
|
postnatal newborn
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Formaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: epidermis cornified layer |
Present |
Regionally restricted |
4_+/+ birth
|
Expression is in the cornified envelope of the ventral skin.
|
Specimen
4 -/- birth:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6N
|
|
Mutant Allele(s):
|
Pparatm1Gonz/Pparatm1Gonz
|
|
Age:
|
postnatal newborn
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Formaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: epidermis cornified layer |
Weak |
Regionally restricted |
4_-/- birth
|
Expression is in the cornified envelope of the ventral skin.
|
Specimen
not shown E17.5 WT:
embryonic day 17.5
(more )
Specimen
not shown E17.5 WT:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6N
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Formaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: epidermis |
Trace |
|
|
The authors state that staining was very faint or absent.
|
Specimen
not shown E17.5-/-:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6N
|
|
Mutant Allele(s):
|
Pparatm1Gonz/Pparatm1Gonz
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Formaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: epidermis |
Trace |
|
|
The authors state that staining was very faint or absent.
|
|
Analysis Tools