Ppara^tm1Gonz
Targeted Allele Detail

Summary

• Symbol: Ppara^tm1Gonz
• Name: peroxisome proliferator activated receptor alpha; targeted mutation 1, Frank J Gonzalez
• MGI ID: MGI:1857774
• Synonyms: mPPARalpha-, PPAR-alpha-, PPARalpha-, PPARalpha-null, Pparat^niJGonz
• Gene: Ppara Location: Chr15:85619184-85687020 bp, + strand Genetic Position: Chr15, 40.42 cM
• Alliance: Ppara^tm1Gonz page

Accumulation of intracellular lipid in the liver of fasted Ppara^tm1Gonz/Ppara^tm1Gonz mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:25516
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette replaced 83 bp of the coding sequence in exon 8. Northern blot analysis did not detect a transcript of wild-type size on RNA derived from liver of homozygous mice. An larger abnormal transcript was detected. Western blot analysis did not detect PPARA protein in extracts derived from livers of homozygous mice. (J:25516, J:91859)

Expression

In Mice Carrying this Mutation:	132 assay results
In Structures Affected by this Mutation:	13 anatomical structures

Tumor Data

List all tumor models in MMHCdb carrying Ppara^tm1Gonz

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 5 strains available Cell Lines: 0 lines available
• Carrying any Ppara Mutation: 32 strains or lines available

References

• Original: J:25516 Lee SS, et al., Targeted disruption of the alpha isoform of the peroxisome proliferator-activated receptor gene in mice results in abolishment of the pleiotropic effects of peroxisome proliferators. Mol Cell Biol. 1995 Jun;15(6):3012-22
• All: 378 reference(s)