Antibody:	Anti-EPCR 1560
Detection system:	Secondary antibody coupled to Texas Red
Assay notes:	Three antibodies were described; the one used in this assay was not specified. MGI:5811364, MGI:5811365, or MGI:581363 could have been used.

Structure	Level	Pattern	Image	Note
TS11: trophoblast giant cell	Present		1A
TS11: embryo	Absent		1A

Structure	Level	Pattern	Image	Note
TS28: vasculature of uterus	Present	Regionally restricted	1A	Expression was detected in endothelial cells lining the venous sinusoids in the uterus.
TS28: venous system endothelium	Present	Regionally restricted	1A	Expression was detected in endothelial cells lining the venous sinusoids in the uterus.
TS28: decidua	Present	Non-Uniform	1A	Decidual cells in the compact layer stained variably, whereas those in the spongy layer exhibited little or no staining.

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		1C
TS11: trophoblast giant cell	Absent		1C

Structure	Level	Pattern	Image	Note
TS28: vasculature of uterus	Present	Regionally restricted	1C	Expression was detected in endothelial cells lining the venous sinusoids in the uterus.
TS28: venous system endothelium	Present	Regionally restricted	1C	Expression was detected in endothelial cells lining the venous sinusoids in the uterus.

Structure	Level	Pattern	Image	Note
TS15: embryo	Absent		1F

Structure	Level	Pattern	Image	Note
TS28: vasculature of uterus	Strong	Regionally restricted	1F	Expression was strong in the endothelial cells lining the venous sinusoids.
TS28: venous system endothelium	Strong	Regionally restricted	1F	Expression was strong in the endothelial cells lining the venous sinusoids.
TS28: decidua	Strong	Regionally restricted	1F	Expression was detected in the decidual cells of the compact layer, but the cells in the spongy layer were negative.

Structure	Level	Pattern	Image	Note
TS21: trophoblast giant cell	Present		2F
TS21: umbilical blood vessel	Absent		2F
TS21: labyrinthine zone	Absent		2F	Staining was absent from Pecam1-positive endothelial cells in the placental labyrinth.

Structure	Level	Pattern	Image	Note
TS22: trophoblast giant cell	Present		2G	Staining increased with time in trophoblasts.
TS22: umbilical blood vessel	Absent		2G
TS22: labyrinthine zone	Absent		2G	Staining was absent from Pecam1-positive endothelial cells in the placental labyrinth.

Structure	Level	Pattern	Image	Note
TS23: trophoblast giant cell	Present		2H	Staining increased with time in trophoblasts.
TS23: umbilical blood vessel	Absent		2H
TS23: labyrinthine zone	Absent		2H	Staining was absent from Pecam1-positive endothelial cells in the placental labyrinth.

Structure	Level	Pattern	Image	Note
TS24: aorta	Weak		3C

Structure	Level	Pattern	Image	Note
TS24: vena cava	Weak		3D

Structure	Level	Pattern	Image	Note
TS26: blood vessel endothelium	Present	Regionally restricted	3E	Expression was detected in the endothelium of some vessels in close proximity to the ribs.
TS26: aorta	Present		3E	Expression was confined to the endothelial cells.
TS26: vena cava	Present		3E	Expression was confined to the endothelial cells.

Structure	Level	Pattern	Image	Note
TS27: aorta	Present	Regionally restricted	3F	Expression was detected in aortic endothelial cells.
TS27: endocardial tissue	Absent		3F
TS27: heart blood vessel	Absent		3F

Structure	Level	Pattern	Image	Note
TS28: aorta endothelium	Present		3G
TS28: vena cava	Present		3G	Expression was detected in endothelial cells.

Structure	Level	Pattern	Image	Note
TS28: endocardial tissue	Present		3H

Structure	Level	Pattern	Image	Note
TS27: lung vascular element	Absent		4A

Structure	Level	Pattern	Image	Note
TS28: lung vascular element	Strong	Regionally restricted	4B	The larger vessels of the lung, but not the alveolar capillaries, stained strongly.

Structure	Level	Pattern	Image	Note
TS27: liver vasculature	Absent		4C

Structure	Level	Pattern	Image	Note
TS28: liver vasculature	Present	Regionally restricted	4D	Expression was detected in the large vessels and sinusoidal capillaries.
TS28: hepatic artery	Present		4D
TS28: hepatic vein	Present	Regionally restricted	4D	Staining was detected in the enothelium of central veins.

Structure	Level	Pattern	Image	Note
TS27: renal artery	Moderate	Regionally restricted	4E	Staining was observed in the largest arteries and veins, but not the smaller vessels.
TS27: renal vein	Moderate	Regionally restricted	4E	Staining was observed in the largest arteries and veins, but not the smaller vessels.
TS27: glomerular capillary system	Absent		4E

Structure	Level	Pattern	Image	Note
TS28: renal artery	Moderate	Regionally restricted	4F	Staining was observed in the largest arteries and veins, but not the smaller vessels.
TS28: renal vein	Moderate	Regionally restricted	4F	Staining was observed in the largest arteries and veins, but not the smaller vessels.
TS28: glomerular capillary system	Absent		4F

Structure	Level	Pattern	Image	Note
TS27: brain blood vessel	Present	Regionally restricted	4G	Expression was detected in the larger vessels, but not capillaries.

Structure	Level	Pattern	Image	Note
TS28: brain blood vessel	Present	Regionally restricted	4H	Expression was detected in the larger vessels, but not capillaries.