Procr^tm1Cte
Targeted Allele Detail

Summary

• Symbol: Procr^tm1Cte
• Name: protein C receptor, endothelial; targeted mutation 1, Charles T Esmon
• MGI ID: MGI:2445432
• Synonyms: EPCR^-
• Gene: Procr Location: Chr2:155593037-155597391 bp, + strand Genetic Position: Chr2, 77.26 cM, cytoband H1-3
• Alliance: Procr^tm1Cte page

Procr^tm1Cte/Procr^tm1Cte embryos are small at E8, growth retarded by E9.5, and do not complete axial rotation

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80043
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exon 1 was flanked by loxP sites and subsequently excised via in vitro cre mediated recombination. The resultant germline allele contained a single loxP site in place of exon 1, which included the 5' untranslated region and encoded the start codon, signal peptide, and part of the extracellular domain. RT-PCR indicated an absence of transcript in homozygous mutant embryos. (J:80043)

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Procr Mutation: 15 strains or lines available

Notes

ES cell line = AB2.

References

• Original: J:80043 Gu JM, et al., Disruption of the endothelial cell protein C receptor gene in mice causes placental thrombosis and early embryonic lethality. J Biol Chem. 2002 Nov 8;277(45):43335-43
• All: 8 reference(s)