Bmp2 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:195918 Vieux-Rochas M, et al., BMP-mediated functional cooperation between Dlx5;Dlx6 and Msx1;Msx2 during mammalian limb development. PLoS One. 2013;8(1):e51700
Assay type:	RT-PCR
MGI Accession ID:	MGI:5810623
Gene symbol:	Bmp2
Gene name:	bone morphogenetic protein 2

Probe:

Bmp2-pY, Bmp2-pZ

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
Msx1-/- HL ant	E11.0	TS18: hindlimb bud	Weak	Not Specified	involves: 129P2/OlaHsd * C57BL/6 * DBA	Dlx5/Dlx6^tm1Levi/Dlx5/Dlx6^tm1Levi, Dlx5/Dlx6^tm1Levi/Dlx5/Dlx6^tm1Levi, Msx1^tm1Bero/Msx1⁺	Not Specified
Msx1-/- HL post	E11.0	TS18: hindlimb bud	Weak	Not Specified	involves: 129P2/OlaHsd * C57BL/6 * DBA	Dlx5/Dlx6^tm1Levi/Dlx5/Dlx6^tm1Levi, Dlx5/Dlx6^tm1Levi/Dlx5/Dlx6^tm1Levi, Msx1^tm1Bero/Msx1⁺	Not Specified
Dlx5-/-;Dlx6-/- HL ant	E11.0	TS18: hindlimb bud	Weak (a)	Not Specified	involves: C57BL/6 * DBA	Dlx5/Dlx6^tm1Levi/Dlx5/Dlx6^tm1Levi, Dlx5/Dlx6^tm1Levi/Dlx5/Dlx6^tm1Levi	Not Specified	This embryo was a single homozygote. However, since this allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animal looking like a double homozygote).
Dlx5-/-;Dlx6-/- HL post	E11.0	TS18: hindlimb bud	Weak (b)	Not Specified	involves: C57BL/6 * DBA	Dlx5/Dlx6^tm1Levi/Dlx5/Dlx6^tm1Levi, Dlx5/Dlx6^tm1Levi/Dlx5/Dlx6^tm1Levi	Not Specified	This embryo was a single homozygote. However, since this allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animal looking like a double homozygote).
Msx1+/-;Dlx5+/-;Dlx5+/- HL ant	E11.0	TS18: hindlimb bud	Trace (c)	Not Specified	involves: 129P2/OlaHsd * C57BL/6 * DBA	Dlx5/Dlx6^tm1Levi/Dlx5⁺, Dlx5/Dlx6^tm1Levi/Dlx6⁺, Msx1^tm1Bero/Msx1⁺	Not Specified	This embryo was a a double heterozygote. However, since the Dlx5/6 allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animal looking like a triple heterozygote).
Msx1+/-;Dlx5+/-;Dlx5+/- HL post	E11.0	TS18: hindlimb bud	Trace (c)	Not Specified	involves: 129P2/OlaHsd * C57BL/6 * DBA	Dlx5/Dlx6^tm1Levi/Dlx5⁺, Dlx5/Dlx6^tm1Levi/Dlx6⁺, Msx1^tm1Bero/Msx1⁺	Not Specified	This embryo was a double heterozygote. However, since the Dlx5/6 allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animal looking like a triple heterozygote).
Msx1+/-;Dlx5-/-;Dlx5-/- HL ant	E11.0	TS18: hindlimb bud	Weak	Not Specified	involves: 129P2/OlaHsd * C57BL/6 * DBA	Dlx5/Dlx6^tm1Levi/Dlx5/Dlx6^tm1Levi, Dlx5/Dlx6^tm1Levi/Dlx5/Dlx6^tm1Levi, Msx1^tm1Bero/Msx1⁺	Not Specified	This embryo was a single homozygote for the Dl5/6 allele. However, since that allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animal looking like a double homozygote).
Msx1+/-;Dlx5-/-;Dlx5-/- HL post	E11.0	TS18: hindlimb bud	Weak	Not Specified	involves: 129P2/OlaHsd * C57BL/6 * DBA	Dlx5/Dlx6^tm1Levi/Dlx5/Dlx6^tm1Levi, Dlx5/Dlx6^tm1Levi/Dlx5/Dlx6^tm1Levi, Msx1^tm1Bero/Msx1⁺	Not Specified	This embryo was a single homozygote for the Dl5/6 allele. However, since that allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animal looking like a double homozygote).

Notes:
(a) Expression decreased in the anterior half by about 50% compared to wildtype.
(b) Expression decreased in the posterior half by about 20% compared to wildtype.
(c) Authors report expression reduced to levels lower than 10% that of control samples.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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