About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:229524 Robert T, et al., The TopoVIB-Like protein family is required for meiotic DNA double-strand break formation. Science. 2016 Feb 26;351(6276):943-9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5796798
Gene symbol: H2ax
Gene name: H2A.X variant histone
Antibody: anti-phosphoH2AX (#05-636; JBW301)
Detection system: Secondary antibody
Results
Specimen 5A +/+ Leptotene: postnatal day 30 (more )
Note: Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present 5A +/+ Leptotene H2afx
Specimen 5A +/+ Zygotene: postnatal day 30 (more )
Note: Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present 5A +/+ Zygotene H2afx
Specimen 5A +/+ Pachytene: postnatal day 30 (more )
Note: Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present 5A +/+ Pachytene H2afx
Specimen 5A -/- Leptotene: postnatal day 30; Top6blem2Arte/Top6blem2Arte (more )
Note: Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Weak 5A -/- Leptotene H2afx
Specimen 5A -/- Zygotene Like: postnatal day 30; Top6blem2Arte/Top6blem2Arte (more )
Note: Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Weak 5A -/- Zygotene like H2afx upper
Specimen 5A -/- Zygotene Like: postnatal day 30; Top6blem2Arte/Top6blem2Arte (more )
Note: Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Weak 5A -/- Zygotene like H2afx lower
Specimen S17A +/+ Leptotene: postnatal month 8 (more )
Note: Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S17A_+/+ Leptotene gH2afx
Specimen S17A +/+ Zygotene: postnatal month 8 (more )
Note: Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S17A_+/+ Zygotene gH2afx
Specimen S17A +/+ Pachytene: postnatal month 8 (more )
Note: Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S17A_+/+ Pachytene gH2afx
Specimen S17A -/- Leptotene: postnatal month 8; Top6blem1Arte/Top6blem2Arte (more )
Note: Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Weak S17A_-/- Leptotene gH2afx
Specimen S17A -/- Zygo. Like: postnatal month 8; Top6blem1Arte/Top6blem2Arte (more )
Note: Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Weak S17A_-/- Zygo. Like gH2afx
Specimen S17A -/- Zygo Like: postnatal month 8; Top6blem1Arte/Top6blem2Arte (more )
Note: Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Weak S17A_-/- Zygo Like gH2afx
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory