Gene Expression Data
Assay Details
Assay
|
Reference:
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J:3158
Lyons GE, et al., Expression of NCAM isoforms during skeletal myogenesis in the mouse embryo. Dev Dyn. 1992 Jun;194(2):94-104
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5796380
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Gene symbol:
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Ncam1
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Gene name:
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neural cell adhesion molecule 1
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Probe:
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NCAM TM probe
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
not shown:
embryonic day 9.0
(more )
Note: 14 somites
Specimen
not shown:
(close )
Genetic Background:
|
either: BALB/c or C3H
|
Age:
|
embryonic day 9.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
14 somites
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: myotome |
Present |
|
|
Expression first detected in myotomes of 14 somite embryos.
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Specimen
1C:
embryonic day 9.0
(more )
Note: 18 somites
Specimen
1C:
(close )
Genetic Background:
|
either: BALB/c or C3H
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Age:
|
embryonic day 9.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
18 somites
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: myotome |
Present |
|
1C
|
Expression specifically in myotomal cells which have formed under the dermatome.
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TS14: neural tube |
Absent |
|
1C
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Expression in the neural tube is at or below the level of detection at this stage.
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Specimen
2A/B:
embryonic day 9.25
(more )
Note: 21-24 somites
Specimen
2A/B:
(close )
Genetic Background:
|
either: BALB/c or C3H
|
Age:
|
embryonic day 9.25
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
21-24 somites
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: somite |
Present |
Scattered |
2A/B
|
Scattered expression over the entire somite.
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TS15: neural tube |
Present |
Diffuse |
2A/B
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Diffuse expression in neural tube at a level roughly comparable to that in the somites.
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TS15: hindgut |
Absent |
|
2A/B
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Authors report there was no expression in other embryonic structures such as the embryonic hindgut.
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Specimen
3C:
embryonic day 10.5
(more )
Note: ~36 somites
Specimen
3C:
(close )
Genetic Background:
|
either: BALB/c or C3H
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
~36 somites
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: myotome |
Present |
Regionally restricted |
3C
|
Expression in a mature myotome near the forelimb bud and more recently formed caudal myotomes.
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TS17: cardiac muscle tissue |
Absent |
|
3C
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No expression in cardiac myocytes.
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TS17: forelimb bud |
Absent |
|
3C
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No expression in myogenic cells migrating into early limb buds.`
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TS17: hindlimb bud |
Absent |
|
3C
|
No expression in myogenic cells migrating into early limb buds.`
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TS17: neural tube |
Strong |
Regionally restricted |
3C
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High level of expression in the ventral root region of the neural tube.
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Specimen
4A/B:
embryonic day 11.5
(more )
Note: 40-48 somites
Specimen
4A/B:
(close )
Genetic Background:
|
either: BALB/c or C3H
|
Age:
|
embryonic day 11.5
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
40-48 somites
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: myotome |
Present |
|
4A/B
|
High level of expression in the ventral root region of the neural tube.
|
TS19: neural tube |
Present |
|
4A/B
|
High level of expression in the ventral root region of the neural tube.
|
Specimen
5A:
embryonic day 12.5
(more )
Specimen
5A:
(close )
Genetic Background:
|
either: BALB/c or C3H
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
6A/B:
embryonic day 13.5
(more )
Specimen
6A/B:
(close )
Genetic Background:
|
either: BALB/c or C3H
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
|