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Gene Expression Data
Assay Details
Assay
Reference: J:37331 Wray S, et al., GABAergic neurons in the embryonic olfactory pit/vomeronasal organ: maintenance of functional GABAergic synapses in olfactory explants. Dev Biol. 1996 Dec 15;180(2):631-45
Assay type: RNA in situ
MGI Accession ID: MGI:5796189
Gene symbol: Gad1
Gene name: glutamate decarboxylase 1
Probe: Gad67 probe7
Probe preparation: Antisense labelled with S35 RNA
Visualized with: Autoradiography
Results
Specimen not shown: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: nasal pit Absent
Specimen 2A E11.5: embryonic day 11.5 (more )
Note: After hybridization, the area of the olfactory pit(OP), the mean optical density (OD), and the area of silver grains within the olfactory pit were measured. The OD/OP was calculated and the changes in relative OD/OP were shown in a graph in Fig. 2A.
Structure Level Pattern Image Note
TS19: nasal pit Weak
Specimen 2A E12.5: embryonic day 12.5 (more )
Note: After hybridization, the area of the olfactory pit(OP), the mean optical density (OD), and the area of silver grains within the olfactory pit were measured. The OD/OP was calculated and the changes in relative OD/OP were shown in a graph in Fig. 2A.
Structure Level Pattern Image Note
TS20: nose Present There was a dramatic increase in expression in the olfactory pit between E11.5 and E12.5.
Specimen 2A E13.5: embryonic day 13.5 (more )
Note: After hybridization, the area of the olfactory pit(OP), the mean optical density (OD), and the area of silver grains within the olfactory pit were measured. The OD/OP was calculated and the changes in relative OD/OP were shown in a graph in Fig. 2A.
Structure Level Pattern Image Note
TS21: nose Present There was a decrease in expression in the olfactory pit between E12.5 and E13.5.
Specimen 2A E16.5: embryonic day 16.5 (more )
Note: After hybridization, the area of the olfactory pit(OP), the mean optical density (OD), and the area of silver grains within the olfactory pit were measured. The OD/OP was calculated and the changes in relative OD/OP were shown in a graph in Fig. 2A.
Structure Level Pattern Image Note
TS24: vomeronasal organ Weak By E16.5 the expression in the olfactory pit/vomeronasal organ was dramatically reduced.
Specimen 2B: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: nasal pit Present 2B Only 2 of 6 animals showed a hybridization signal over the olfatory pit region.
TS19: telencephalon Present 2B All animals showed staining in the telencephalon in the region of the lamina terminalis/optica chiasima.
Specimen 2C: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: vomeronasal organ Present Regionally restricted 2C Expression was detected in cells in the ventral aspect of the olfactory pit/vomeronasal organ.
TS21: olfactory epithelium Present Regionally restricted 2C Expression was detected in the ventral olfactory epithelium. Expression was much less than that seen in the developing brain. No expression was detected along olfactory axons or outside the epithelium.
TS21: brain Present 2C
Specimen 2D: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: vomeronasal organ epithelium Present Regionally restricted 2D Expression was decreased compared to earlier stages but remained in a small subset of respiratory epithelial cells
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory