Assay
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Reference:
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J:229480
Baker JL, et al., Testicular receptor 2, Nr2c1, is associated with stem cells in the developing olfactory epithelium and other cranial sensory and skeletal structures. Gene Expr Patterns. 2016 Jan;20(1):71-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5795684
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Gene symbol:
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Nr2c1
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Gene name:
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nuclear receptor subfamily 2, group C, member 1
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Results
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Specimen
2A/C/D/E:
embryonic day 10.5
(more )
Note: Double labeled: red - Nr2c1; green - Pax7 (assay MGI:5795696).
Specimen
2A/C/D/E:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 10.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Double labeled: red - Nr2c1; green - Pax7 (assay MGI:5795696).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: olfactory epithelium |
Present |
Punctate |
2E
,
2D
,
2C
,
2A
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Concentrated in puncta within the nucleus. A subset of Nr2c1-expressing cells coexpress Pax7.
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Specimen
2F/H/I/J:
embryonic day 10.5
(more )
Note: Double labeled: red - Nr2c1; green - Ascl1 (assay MGI:5795697).
Specimen
2F/H/I/J:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
|
Note:
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Double labeled: red - Nr2c1; green - Ascl1 (assay MGI:5795697).
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Specimen
2K/L/O:
embryonic day 10.5
(more )
Note: Double labeled: red - Nr2c1; green - Ncam1 (assay MGI:5795700).
Specimen
2K/L/O:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
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Double labeled: red - Nr2c1; green - Ncam1 (assay MGI:5795700).
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Specimen
3A/C/D:
embryonic day 11.5
(more )
Note: Double labeled: red - Nr2c1; green - Pax7 (assay MGI:5795696).
Specimen
3A/C/D:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
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Double labeled: red - Nr2c1; green - Pax7 (assay MGI:5795696).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: olfactory epithelium |
Present |
Widespread |
3C
,
3A
,
3D
|
Expressed throughout, but more robust in the medial versus lateral region. No longer robustly associated with Pax7 labeled olfactory stem cells.
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Specimen
3F/H/I/J:
embryonic day 11.5
(more )
Note: Double labeled: red - Nr2c1; green - Ascl1 (assay MGI:5795697).
Specimen
3F/H/I/J:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
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Double labeled: red - Nr2c1; green - Ascl1 (assay MGI:5795697).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: olfactory epithelium |
Present |
Widespread |
3I
,
3J
,
3F
,
3H
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Expressed throughout, but more robust in the medial versus lateral region. In the medial olfactory epithelium frequently coexpressed with Ascl1. Expression is primarily cytoplasmic.
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Specimen
3K/M/N/P:
embryonic day 11.5
(more )
Note: Double labeled: red - Nr2c1; green - Ncam1 (assay MGI:5795700).
Specimen
3K/M/N/P:
(close )
Genetic Background:
|
CD-1
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Age:
|
embryonic day 11.5
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Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Nr2c1; green - Ncam1 (assay MGI:5795700).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: olfactory epithelium |
Present |
Widespread |
3N
,
3P
,
3M
,
3K
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Expressed throughout, but more robust in the medial versus lateral region. Most Ncam1 positive cells do not express Nr2c1.
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Specimen
4A/B:
embryonic day 17.5
(more )
Specimen
4A/B:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: olfactory epithelium |
Strong |
Widespread |
4A
,
4B
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Expressed in both the thicker, presumptive neural epithelium, and in the thinner, presumptive respiratory epithelium. Primarily cytoplasmic.
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Specimen
4C:
embryonic day 16.5
(more )
Note: Double labeled: red - Nr2c1; green - Ascl1 (assay MGI:5795697).
Specimen
4C:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Nr2c1; green - Ascl1 (assay MGI:5795697).
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Specimen
4D:
embryonic day 14.5
(more )
Specimen
4D:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4E:
embryonic day 17.5
(more )
Specimen
4E:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4F:
embryonic day 11.5
(more )
Note: Double labeled: red - Nr2c1; green - Nes (assay MGI:5795703).
Specimen
4F:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Nr2c1; green - Nes (assay MGI:5795703).
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Specimen
4G:
embryonic day 14.5
(more )
Note: Double labeled: red - Nr2c1; green - Nes (assay MGI:5795703).
Specimen
4G:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Nr2c1; green - Nes (assay MGI:5795703).
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Specimen
5A/B:
embryonic day 10.5
(more )
Note: Double labeled: red - Nr2c1; green - Tubb3 (assay MGI:5795701).
Specimen
5A/B:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Nr2c1; green - Tubb3 (assay MGI:5795701).
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Specimen
5C:
embryonic day 16.5
(more )
Note: Double labeled: red - Nr2c1; green - Nes (assay MGI:5795703).
Specimen
5C:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 16.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
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Double labeled: red - Nr2c1; green - Nes (assay MGI:5795703).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: vibrissa |
Strong |
Regionally restricted |
5C
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Heavily expressed in the supporting cells of the mystacial vibrissae.
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Specimen
5D:
embryonic day 16.5
(more )
Note: Double labeled: red - Nr2c1; green - Ncam1 (assay MGI:5795700).
Specimen
5D:
(close )
Genetic Background:
|
CD-1
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Age:
|
embryonic day 16.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Nr2c1; green - Ncam1 (assay MGI:5795700).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: vibrissa |
Strong |
Regionally restricted |
5D
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Heavily expressed in the supporting cells of the mystacial vibrissae. But not coincided with the NCAM1-positive neural processes that innervate the vibrissae.
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Specimen
5E:
embryonic day 14.5
(more )
Specimen
5E:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6A/B/C:
embryonic day 17.5
(more )
Note: Double labeled: red - Nr2c1; green - Nes (assay MGI:5795703).
Specimen
6A/B/C:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Nr2c1; green - Nes (assay MGI:5795703).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: tooth mesenchyme |
Strong |
Regionally restricted |
6A
,
6B
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Expressed in the dental pulp. Expressed in the vicinity of, and co-localized with a subset of Nestin-positive presumptive dental mesenchymal stem cells.
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TS25: tooth |
Strong |
Regionally restricted |
6A
,
6C
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Expressed in a subset of presumptive odontoblasts at the margin of the dental pulp.
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Specimen
6D:
embryonic day 17.5
(more )
Specimen
6D:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS25: jaw |
Present |
Regionally restricted |
6D
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Expressed in the mesenchymal stem cells and presumptive osteoblasts in the developing alveolar bone.
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Specimen
6E/F/G:
embryonic day 17.5
(more )
Specimen
6E/F/G:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S1C:
embryonic day 17.5
(more )
Note: Double labeled: red - Nr2c1; green - Nes (assay MGI:5795703).
Specimen
S1C:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 17.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Nr2c1; green - Nes (assay MGI:5795703).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: head |
Strong |
|
S1C
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Expressed in multiple craniofacial structures.
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Specimen
not shown:
embryonic day 10.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS17: forebrain |
Trace |
Diffuse |
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Limited hardly above background expression in the cortex, ventral forebrain structures, and thalamus.
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Specimen
not shown:
embryonic day 14.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: forebrain |
Trace |
Diffuse |
|
Limited hardly above background expression in the cortex, ventral forebrain structures, and thalamus.
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TS22: cerebral cortex |
Trace |
Diffuse |
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Limited hardly above background expression.
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TS22: thalamus |
Trace |
Diffuse |
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Limited hardly above background expression.
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