Gene Expression Data
Assay Details
Assay
|
Reference:
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J:178320
Loebel DA, et al., Rhou maintains the epithelial architecture and facilitates differentiation of the foregut endoderm. Development. 2011 Oct;138(20):4511-22
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5790697
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Gene symbol:
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Rhou
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Gene name:
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ras homolog family member U
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Probe:
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Rhou probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 8.0
(more )
Note: 3-somite stage
Specimen
1A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
|
Note:
|
3-somite stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: foregut diverticulum |
Present |
Regionally restricted |
1A
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Expression was strongest in the rim of the foregut pocket, where the liver progenitors reside.
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Specimen
1B:
embryonic day 8.0
(more )
Note: 3-somite stage
Specimen
1B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
3-somite stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: foregut diverticulum |
Present |
Regionally restricted |
1B
|
Expression was strongest in the rim of the foregut pocket, where the liver progenitors reside.
|
Specimen
1C:
embryonic day 8.5
(more )
Note: 8-somite stage
Specimen
1C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
8-somite stage
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Specimen
1C(i):
embryonic day 8.5
(more )
Note: 8-somite stage
Specimen
1C(i):
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
8-somite stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: foregut diverticulum |
Strong |
Regionally restricted |
1C(i)
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Expression was strong in the columnar epithelium on the lateral wall and the floor of the foregut but weak in the thin squamous epithelium of the roof.
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Specimen
1C(ii):
embryonic day 8.5
(more )
Note: 8-somite stage
Specimen
1C(ii):
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
8-somite stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: foregut diverticulum |
Strong |
Regionally restricted |
1C(ii)
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Expression was strong in the columnar epithelium on the lateral wall and the floor of the foregut but weak in the thin squamous epithelium of the roof.
|
Specimen
1C(iii):
embryonic day 8.5
(more )
Note: 8-somite stage
Specimen
1C(iii):
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
8-somite stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: foregut diverticulum |
Present |
Regionally restricted |
1C(iii)
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Expressed throughout the foregut except the regions anterior to the first branchial arch and the ventral lip of the anterior intestinal portal.
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Specimen
1D:
embryonic day 9.5
(more )
Specimen
1D:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1D(i):
embryonic day 9.5
(more )
Specimen
1D(i):
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: branchial pouch |
Present |
|
1D(i)
|
|
TS15: gut endoderm |
Present |
Regionally restricted |
1D(i)
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Expressed in the endoderm on the lateral wall of the caudal segment of the anterior intestinal portal.
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TS15: foregut epithelium |
Weak |
Regionally restricted |
1D(i)
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Expressed in the thickened epithelium on the floor of the foregut where the thyroid primordium emerges.
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Specimen
1D(ii):
embryonic day 9.5
(more )
Specimen
1D(ii):
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2D:
embryonic day 8.5
(more )
Specimen
2D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Specimen
2E:
embryonic day 8.5
(more )
Specimen
2E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Specimen
2F:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Dkk1tm1Lmgd/Dkk1tm1Lmgd
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Age:
|
embryonic day 8.5
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Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: foregut diverticulum |
Present |
|
2F
|
Increased expression in the foregut and adjacent tissues, compared with wild-type.
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Specimen
2G:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
|
Dkk1tm1Lmgd/Dkk1tm1Lmgd
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Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: foregut diverticulum |
Present |
|
2G
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Increased expression in the foregut and adjacent tissues, compared with wild-type.
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Specimen
not shown:
embryonic day 10.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
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