Antibody:	anti-Sert
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb external plexiform layer	Strong		3A
TS28: main olfactory bulb glomerular layer	Strong		3A
TS28: main olfactory bulb granule cell layer	Strong		3A
TS28: main olfactory bulb internal plexiform layer	Strong		3A
TS28: main olfactory bulb mitral cell layer	Strong		3A

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb external plexiform layer	Present		3B	Fewer serotonergic fibers, compared with the wild-type.
TS28: main olfactory bulb glomerular layer	Present		3B	Fewer serotonergic fibers, compared with the wild-type.
TS28: main olfactory bulb internal plexiform layer	Present		3B	Fewer serotonergic fibers, compared with the wild-type.
TS28: main olfactory bulb mitral cell layer	Present		3B	Fewer serotonergic fibers, compared with the wild-type.
TS28: main olfactory bulb granule cell layer	Strong		3B	More serotonergic fibers, compared with the wild-type.

Structure	Level	Pattern	Image	Note
TS28: cingulate cortex	Present		3C

Structure	Level	Pattern	Image	Note
TS28: cingulate cortex	Present		3D	Lower expression throughout, compared with wild-type mice.

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Strong		3E	With a slightly higher density in layers I and IV.
TS28: cortical layer II	Strong		3E	With a slightly higher density in layers I and IV.
TS28: cortical layer III	Strong		3E	With a slightly higher density in layers I and IV.
TS28: cortical layer IV	Strong		3E	With a slightly higher density in layers I and IV.
TS28: cortical layer V	Strong		3E	With a slightly higher density in layers I and IV.
TS28: cortical layer VI	Strong		3E	With a slightly higher density in layers I and IV.

Structure	Level	Pattern	Image	Note
TS28: cortical layer I	Strong		3F	More serotonergic fibers, compared with the wild-type.
TS28: cortical layer II	Present	Regionally restricted	3F	Short, clumped fibers were frequently observed in layer II/III of only the most rostral part of the primary motor cortex.
TS28: cortical layer III	Present	Regionally restricted	3F	Short, clumped fibers were frequently observed in layer II/III of only the most rostral part of the primary motor cortex.

Structure	Level	Pattern	Image	Note
TS28: entorhinal cortex	Present		3G
TS28: dorsal lateral geniculate nucleus	Present	Uniform	3G

Structure	Level	Pattern	Image	Note
TS28: entorhinal cortex	Present		3H	Dramatically greater expression than in wild-type mice.
TS28: dorsal lateral geniculate nucleus	Present	Regionally restricted	3H	Serotonergic fibers were accumulated around these nuclei and sparse in the central regions.

Structure	Level	Pattern	Image	Note
TS28: caudate-putamen	Moderate		4A
TS28: globus pallidus	Strong	Uniform	4A	Fairly evenly distributed.

Structure	Level	Pattern	Image	Note
TS28: caudate-putamen	Weak		4B	Dramatically decreased expression, compared with the wild-type.
TS28: globus pallidus	Strong		4B	Increased expression, prominent only in the peripheral region, compared with the wild-type. The central regions were more sparsely innervated.

Structure	Level	Pattern	Image	Note
TS28: substantia nigra	Present	Widespread	4C

Structure	Level	Pattern	Image	Note
TS28: substantia nigra	Present	Regionally restricted	4D	Obviously increased expression in the peripheral region, compared with the wild-type. The central regions were more sparsely innervated.

Structure	Level	Pattern	Image	Note
TS28: dorsal lateral geniculate nucleus	Present	Uniform	4E
TS28: medial geniculate nucleus	Present	Uniform	4E

Structure	Level	Pattern	Image	Note
TS28: dorsal lateral geniculate nucleus	Present		4F	Serotonergic fibers were accumulated around these nuclei and sparse in the central regions.
TS28: medial geniculate nucleus	Present		4F	Serotonergic fibers were accumulated around these nuclei and sparse in the central regions.

Structure	Level	Pattern	Image	Note
TS28: hippocampus molecular layer	Present		5C , 5A	Relatively higher expression.
TS28: hippocampus stratum lacunosum	Present		5C , 5A	Relatively higher expression.
TS28: hippocampus stratum radiatum	Present		5C , 5A	Relatively higher expression.
TS28: dentate gyrus	Present		5A , 5C	Relatively lower expression.
TS28: hippocampus stratum oriens	Present		5A , 5C	Relatively lower expression.

Structure	Level	Pattern	Image	Note
TS28: hippocampus molecular layer	Present		5B , 5D	Dramatically increased expression, compared with wild-type. A quantitative analysis was shown in 5F.
TS28: hippocampus stratum lacunosum	Present		5B , 5D	Dramatically increased expression, compared with wild-type. A quantitative analysis was shown in 5F.
TS28: dentate gyrus	Present		5B , 5D	Decreased expression, compared with wild-type. A quantitative analysis was shown in 5F.
TS28: hippocampus stratum oriens	Present		5B , 5D	Decreased expression, compared with wild-type. A quantitative analysis was shown in 5F.
TS28: hippocampus	Present		5B , 5D	There was no significant difference in the average density of the serotonergic fibers as a whole, compared with the wild-type as shown in 5F.

Structure	Level	Pattern	Image	Note
TS28: caudate-putamen	Present	Scattered	6A	Sparse expression.
TS28: globus pallidus	Present	Scattered	6A	Sparse expression.
TS28: telencephalon	Present	Regionally restricted	6A	Some concentration was seen at the border between the caudate-putamen and globus pallidus.

Structure	Level	Pattern	Image	Note
TS28: caudate-putamen	Present	Scattered	6B	Sparse expression, similar as the wild-type.
TS28: globus pallidus	Present	Scattered	6B	Sparse expression, similar as the wild-type.

Structure	Level	Pattern	Image	Note
TS28: dorsal lateral geniculate nucleus	Present	Regionally restricted	6C	Expression was sparse in the central regions and concentrated in the peripheral regions.
TS28: medial geniculate nucleus	Present	Regionally restricted	6C	Expression was sparse in the central regions and concentrated in the peripheral regions.
TS28: substantia nigra	Present	Regionally restricted	6C	Expression was sparse in the central regions and concentrated in the peripheral regions.

Structure	Level	Pattern	Image	Note
TS28: dorsal lateral geniculate nucleus	Present	Regionally restricted	6D	Expression was sparse in the central regions and concentrated in the peripheral regions.
TS28: medial geniculate nucleus	Present	Regionally restricted	6D	Expression was sparse in the central regions and concentrated in the peripheral regions.
TS28: substantia nigra	Present	Regionally restricted	6D	Expression was sparse in the central regions and concentrated in the peripheral regions.

Structure	Level	Pattern	Image	Note
TS28: dorsal lateral geniculate nucleus	Present	Regionally restricted	6E	Expression was sparse in the central regions and concentrated in the peripheral regions.
TS28: medial geniculate nucleus	Present	Regionally restricted	6E	Expression was sparse in the central regions and concentrated in the peripheral regions.
TS28: substantia nigra	Present	Regionally restricted	6E	Expression was sparse in the central regions and concentrated in the peripheral regions.

Structure	Level	Pattern	Image	Note
TS28: dorsal lateral geniculate nucleus	Present	Regionally restricted	6F	Expression was sparse in the central regions and concentrated in the peripheral regions.
TS28: medial geniculate nucleus	Present	Regionally restricted	6F	Expression was sparse in the central regions and concentrated in the peripheral regions.
TS28: substantia nigra	Present	Regionally restricted	6F	Expression was sparse in the central regions and concentrated in the peripheral regions.

Structure	Level	Pattern	Image	Note
TS27: hippocampus molecular layer	Present		6G	Dense expression.
TS27: hippocampus stratum lacunosum	Present		6G	Dense expression.
TS27: hippocampus stratum radiatum	Present		6G	Dense expression.
TS27: hippocampus stratum oriens	Present	Scattered	6G	Sparse expression.
TS27: dentate gyrus	Present	Single cells	6G	Only a few were detected.

Structure	Level	Pattern	Image	Note
TS27: hippocampus molecular layer	Present		6H	Dense expression. Slightly higher than in wild-type.
TS27: hippocampus stratum lacunosum	Present		6H	Dense expression. Slightly higher than in wild-type.
TS27: hippocampus stratum radiatum	Present		6H	Dense expression. Slightly higher than in wild-type.
TS27: dentate gyrus	Present	Scattered	6H	Sparse expression.
TS27: hippocampus stratum oriens	Present	Scattered	6H	Sparse expression.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		7A
TS28: hippocampus molecular layer	Present		7A
TS28: hippocampus stratum lacunosum	Present		7A

Structure	Level	Pattern	Image	Note
TS28: hippocampus molecular layer	Present		7B	Higher expression (170%, summarized in 7D) compared with wild-type.
TS28: hippocampus stratum lacunosum	Present		7B	Higher expression (170%, summarized in 7D) compared with wild-type.
TS28: dentate gyrus	Present		7B	Lower expression (41%, summarized in 7D) compared with wild-type.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		7C	Expression was similar to the wild-type.
TS28: hippocampus molecular layer	Present		7C	Expression was similar to the wild-type.
TS28: hippocampus stratum lacunosum	Present		7C	Expression was similar to the wild-type.

Structure	Level	Pattern	Image	Note
TS28: barrel cortex	Present		S6A

Structure	Level	Pattern	Image	Note
TS28: barrel cortex	Present		S6B	Similar to the wild-type.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present			There was no apparent difference in serotonergic fiber density, compared with wild-type, as shown in 5F.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present			Expression was similar to the wild-type.
TS28: hippocampus molecular layer	Present			Expression was similar to the wild-type.
TS28: hippocampus stratum lacunosum	Present			Expression was similar to the wild-type.