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Gene Expression Data
Assay Details
Assay
Reference: J:198275 Watanabe N, et al., The REV7 subunit of DNA polymerase zeta is essential for primordial germ cell maintenance in the mouse. J Biol Chem. 2013 Apr 12;288(15):10459-71
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5788865
Gene symbol: Mad2l2
Gene name: MAD2 mitotic arrest deficient-like 2
Antibody: Anti-Rev7
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2D: postnatal day 56 (more )
Structure Cell Type Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 2D top right
TS28: spermatocyte Present 2D top right
TS28: spermatogonium Present 2D top right
TS28: spermatozoon Weak 2D top right
TS28: Sertoli cell Sertoli cell Trace 2D top right Expression was almost undetectable.
Specimen 5D: embryonic day 13.5 (more )
Note: Double labeled: green - Mad2l2; brown - Pou5f1 (assay MGI:5788922).
Structure Level Pattern Image Note
TS21: urogenital ridge Present Widespread 5D Merge , 5D Rev7 Expression was detected in most of the cells in the genital ridge, and some were Pou5f1-immunopositive primordial germ cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory