Gene Expression Data
Assay Details
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Assay
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Reference:
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J:198275
Watanabe N, et al., The REV7 subunit of DNA polymerase zeta is essential for primordial germ cell maintenance in the mouse. J Biol Chem. 2013 Apr 12;288(15):10459-71
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5788922
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Gene symbol:
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Pou5f1
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Gene name:
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POU domain, class 5, transcription factor 1
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Antibody:
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Anti-Oct4
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
5A E8.5 Rev7+/+:
embryonic day 8.5
(more  )
Specimen
5A E8.5 Rev7+/+:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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10% Formalin
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Specimen
5A E8.5 Rev7-/-:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Mad2l2tm1Ymu/Mad2l2tm1Ymu
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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10% Formalin
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Specimen
5A E9.5 Rev7+/+:
embryonic day 9.5
(more )
Specimen
5A E9.5 Rev7+/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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10% Formalin
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Specimen
5A E9.5 Rev7-/-:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Mad2l2tm1Ymu/Mad2l2tm1Ymu
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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10% Formalin
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Specimen
5A E10.5 Rev7+/+:
embryonic day 10.5
(more )
Specimen
5A E10.5 Rev7+/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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10% Formalin
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Specimen
5A E10.5 Rev7-/-:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Mad2l2tm1Ymu/Mad2l2tm1Ymu
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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10% Formalin
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Specimen
5B E11.5 Rev7+/+:
embryonic day 11.5
(more )
Specimen
5B E11.5 Rev7+/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Specimen
5B E11.5 Rev7-/-:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Mad2l2tm1Ymu/Mad2l2tm1Ymu
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: primordial germ cell of gonad |
Present |
Single cells |
5B Oct4 E11.5 Rev7-/-
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Expression was detected in a few remaining primordial germ cells around the genital ridges. Comparison with a serial section indicated some cells were also immunopositive for cleaved-Casp3.
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Specimen
5B E13.5 Rev7+/+:
embryonic day 13.5
(more )
Specimen
5B E13.5 Rev7+/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Specimen
5B E13.5 Rev7-/-:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Mad2l2tm1Ymu/Mad2l2tm1Ymu
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Specimen
5D:
embryonic day 13.5
(more )
Note: Double labeled: brown - Pou5f1; green - Mad2l2 (assay MGI:5788865).
Specimen
5D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - Pou5f1; green - Mad2l2 (assay MGI:5788865).
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Specimen
7A Rev7+/+:
embryonic day 9.5
(more )
Specimen
7A Rev7+/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Specimen
7A Rev7-/-:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Mad2l2tm1Ymu/Mad2l2tm1Ymu
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Specimen
7B Rev7+/+:
embryonic day 13.5
(more )
Specimen
7B Rev7+/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Specimen
7B Rev7-/-:
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Mad2l2tm1Ymu/Mad2l2tm1Ymu
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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