Probe:	Sp8 probe2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: telencephalon ventricular layer	Present	Graded	3A	Expressed in a high to low medial to lateral gradient across the dorsal telencephalon ventricular zone.
TS17: telencephalon	Present	Graded	3A	Expressed across the A-P axis of dorsal telencephalon, with a shallow high to low A-P gradient. Also expressed robustly in the lateral ganglionic eminence and at lower levels in the medial ganglionic eminence.
TS17: brain floor plate	Present	Regionally restricted	3A	Expression overlaps with the Fgf8 expression domain in the commissural plate.

Structure	Level	Pattern	Image	Note
TS17: telencephalon ventricular layer	Present	Graded	3B	Expressed in a high to low medial to lateral gradient across the dorsal telencephalon ventricular zone.
TS17: telencephalon	Present	Graded	3B	Expressed across the A-P axis of dorsal telencephalon, with a shallow high to low A-P gradient. Also expressed robustly in the lateral ganglionic eminence and at lower levels in the medial ganglionic eminence.
TS17: brain floor plate	Present	Regionally restricted	3B	Expression overlaps with the Fgf8 expression domain in the commissural plate.

Structure	Level	Pattern	Image	Note
TS19: telencephalon ventricular layer	Present	Graded	3D	Expressed in a high to low medial to lateral gradient across the dorsal telencephalon ventricular zone.
TS19: telencephalon	Present	Graded	3D	Expressed across the A-P axis of dorsal telencephalon, with a shallow high to low A-P gradient.
TS19: brain floor plate	Strong	Regionally restricted	3D	Expression largely excluded from the midline (Fgf8 domain), and expressed in a high to low gradient extending laterally from the midline.

Structure	Level	Pattern	Image	Note
TS19: telencephalon ventricular layer	Present	Graded	3E	Expressed in a high to low medial to lateral gradient across the dorsal telencephalon ventricular zone.
TS19: telencephalon	Present	Graded	3E	Expressed across the A-P axis of dorsal telencephalon, with a shallow high to low A-P gradient. Also expressed robustly in the lateral ganglionic eminence.
TS19: brain floor plate	Strong	Regionally restricted	3E	Expression largely excluded from the midline (Fgf8 domain), and expressed in a high to low gradient extending laterally from the midline.

Structure	Level	Pattern	Image	Note
TS21: telencephalon ventricular layer	Present	Graded	3G	Expressed in a high to low medial to lateral gradient across the dorsal telencephalon ventricular zone. Expression was substantially diminished.
TS21: telencephalon	Present	Graded	3G	Expressed across the A-P axis of dorsal telencephalon, with a shallow high to low A-P gradient. Expression was excluded from the increasingly restricted Fgf8 domain in the commissural plate.

Structure	Level	Pattern	Image	Note
TS21: telencephalon ventricular layer	Present	Graded	3H	Expressed in a high to low medial to lateral gradient across the dorsal telencephalon ventricular zone.
TS21: telencephalon	Present	Graded	3H	Expressed across the A-P axis of dorsal telencephalon, with a shallow high to low A-P gradient. Expression was excluded from the increasingly restricted Fgf8 domain in the commissural plate.
TS21: lateral ganglionic eminence	Strong		3H

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Graded	3J	Expressed across the A-P axis of dorsal telencephalon, with a shallow high to low A-P gradient.
TS15: telencephalon floor plate	Present	Regionally restricted	3J	Expression overlaps with the Fgf8 expression domain in the commissural plate.

Structure	Level	Pattern	Image	Note
TS15: telencephalon	Present	Graded		Expressed in a high to low medial to lateral gradient across the dorsal telencephalon ventricular zone. Expressed across the A-P axis of dorsal telencephalon, with a shallow high to low A-P gradient.
TS15: telencephalon floor plate	Present	Regionally restricted		Expression overlaps with the Fgf8 expression domain in the commissural plate.