Probe:	Melk cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Present	Uniform	2A	Evenly distributed throughout the cytoplasm.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage conceptus	Absent		2B

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage conceptus	Weak	Widespread	2C	Expressed in all cells.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		2D	Restricted in the inner cell mass.
TS4: trophectoderm	Absent		2D

Structure	Level	Pattern	Image	Note
TS15: tail somite	Present	Regionally restricted	3C	Expressed in the ventral portion.

Structure	Level	Pattern	Image	Note
TS17: tail	Present	Regionally restricted	3D	Expressed in the tip of the tail.
TS17: limb bud	Present		3D

Structure	Level	Pattern	Image	Note
TS19: tail	Present	Regionally restricted	3E	Expressed in the tip of the tail.
TS19: eye epithelium	Present		3E
TS19: limb ectoderm	Present		3E
TS19: nasal pit epithelium	Present		3E

Structure	Cell Type	Level	Pattern	Image	Note
TS28: seminiferous tubule		Strong	Regionally restricted	4A	Expressed in the layers containing mainly spermatogonia.
TS28: spermatogonium		Strong		4A
TS28: interstitium of the testis		Absent		4A
TS28: spermatid		Absent		4A
TS28: spermatozoon		Absent		4A
TS28: Leydig cell	Leydig cell	Absent		4A

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present		4B	Expressed in all stages.
TS28: ovary follicle cell	Absent		4B
TS28: mesenchymal stroma of ovary	Weak		4B

Structure	Level	Pattern	Image	Note
TS17: limb ectoderm	Present
TS17: limb mesenchyme	Absent

Structure	Level	Pattern	Image	Note
TS19: eye epithelium	Present
TS19: limb ectoderm	Present
TS19: nasal pit epithelium	Present