Gene Expression Data
Assay Details
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Assay
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Reference:
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J:225923
Potts KS, et al., Mouse prenatal platelet-forming lineages share a core transcriptional program but divergent dependence on MPL. Blood. 2015 Aug 6;126(6):807-16
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5784660
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Gene symbol:
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Gp5
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Gene name:
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glycoprotein 5 platelet
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Results
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Specimen
1B embryo proper:
embryonic day 9.5
(more  )
Note: Triple labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659); blue - VECAD (Cdh5 assay MGI:5784661). Also stained with DAPI (gray).
Specimen
1B embryo proper:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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Triple labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659); blue - VECAD (Cdh5 assay MGI:5784661). Also stained with DAPI (gray).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: mesenchyme derived from splanchnopleure |
Present |
Regionally restricted |
1B embryo proper
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Expression in diploid platelet-forming cells in the splanchnic mesenchyme.
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| TS15: hepatic primordium |
Present |
Regionally restricted |
1B embryo proper
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Expression occasionally within the mesenchyme of the developing liver bud.
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Specimen
1B Placenta:
embryonic day 9.5
(more )
Note: Triple labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659); blue - VECAD (Cdh5 assay MGI:5784661). Also stained with DAPI (gray).
Specimen
1B Placenta:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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Triple labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659); blue - VECAD (Cdh5 assay MGI:5784661). Also stained with DAPI (gray).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: placenta |
Present |
Regionally restricted |
1B Placenta
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Expression in diploid platelet-forming cells in the placental vasculature.
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Specimen
2Ai:
embryonic day 10.5
(more )
Note: Triple labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659); blue - VECAD (Cdh5 assay MGI:5784661).
Specimen
2Ai:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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Triple labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659); blue - VECAD (Cdh5 assay MGI:5784661).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: conceptus |
Present |
Widespread |
2A i
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Expression in cells distributed throughout the conceptus.
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| TS17: head |
Present |
Regionally restricted |
2A i
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Expression in diploid platelet-forming cells in the head vasculature.
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| TS17: liver |
Present |
Regionally restricted |
2A i
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Expression in diploid platelet-forming cells occasionally within the mesenchyme of the developing liver bud.
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Specimen
2Aii:
embryonic day 10.5
(more )
Note: Double labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659).
Specimen
2Aii:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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Double labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: liver |
Present |
Regionally restricted |
2A ii
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Expression in diploid platelet-forming cells occasionally within the mesenchyme of the developing liver bud.
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Specimen
2Aiii:
embryonic day 10.5
(more )
Note: Double labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659).
Specimen
2Aiii:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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Double labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: placenta |
Present |
Regionally restricted |
2A iii
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Expression in diploid platelet-forming cells in the placental vasculature.
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Specimen
3H:
embryonic day 13.5
(more )
Note: Triple labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659); gray - DAPI.
Specimen
3H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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Triple labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659); gray - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: liver |
Present |
Regionally restricted |
3H
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Expression in megakaryocytes.
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Specimen
5Ci:
embryonic day 10.5
(more )
Note: Double labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659).
Specimen
5Ci:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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Double labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: yolk sac |
Present |
Regionally restricted |
5C i
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Expression in megakaryocytes and platelets.
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Specimen
5Ciii Nfe2-/-:
embryonic day 10.5; Nfe2tm1Sho/ Nfe2tm1Sho
(more )
Note: Double labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659).
Specimen
5Ciii Nfe2-/-:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Nfe2tm1Sho/Nfe2tm1Sho
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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Double labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: yolk sac |
Present |
Regionally restricted |
5C iii
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Expression in megakaryocytes.
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Specimen
6C Mpl-/-:
embryonic day 10.5; Mpltm1Wsa/ Mpltm1Wsa
(more )
Note: Double labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659).
Specimen
6C Mpl-/-:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Mpltm1Wsa/Mpltm1Wsa
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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Double labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: yolk sac |
Present |
Regionally restricted |
6C
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Expression in megakaryocytes and platelets.
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Specimen
S2:
embryonic day 9.5
(more )
Note: Triple labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659); blue - VECAD (Cdh5 assay MGI:5784661).
Specimen
S2:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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Note:
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Triple labeled: green - CD42D (Gp5); red - CD41 (Itga2b assay MGI:5784659); blue - VECAD (Cdh5 assay MGI:5784661).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: head |
Present |
Regionally restricted |
S2
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Expression in diploid platelet-forming cells in the head vasculature,
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| TS15: mesenchyme derived from splanchnopleure |
Present |
Regionally restricted |
S2
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Expression in diploid platelet-forming cells in the splanchnic mesenchyme.
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| TS15: hepatic primordium |
Present |
Regionally restricted |
S2
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Expression occasionally within the mesenchyme of the developing liver bud.
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Analysis Tools