Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | Notes | References

Symbol:	Col1a1tm1(tetO-cas9)Sho
Name:	collagen, type I, alpha 1; targeted mutation 1, Stuart Orkin
MGI ID:	MGI:5781096
Gene:	Col1a1  Location: Chr11:94827050-94843868 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance:	Col1a1tm1(tetO-cas9)Sho page

Germline Transmission:	Earliest citation of germline transmission: J:101977
Parent Cell Line:	KH2 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Allele Type:    Targeted (Endonuclease, Inducible) Inducer:    doxycycline/tetracycline Mutation:    Insertion   Mutation details: KH2 embryonic stem cells (C57BL/6 x 129S4Sv/Jae), carrying the Gt(ROSA)26Sor allele were re-targeted to insert an FRT site-flanked PGK-Neo cassette and a promoterless hygromycin resistance cassette downstream of the Col1a1 locus. Correctly re-targeted ES cells were selected and the FRT site-flanked PGK-Neo downstream of the Col1a1 locus was replaced by electroporating both a "flip-in plasmid" containing a PGK promoter, ATG start codon, FRT site, a splice acceptor-double polyA sequence (SA-2xpA), tetO (tetracycline responsive element) with a CMV minimal promoter, and SV40 polyA signal, upstream of the hCas9 (human codon optimized Cas9 nuclease) sequence, and a FLPe recombinase expressing vector to facilitate tetO-hCAS9 recombination into the 3' UTR of the Col1a1 locus. (J:101977)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:	160 strains or lines available

Original:	J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:	3 reference(s)