Gene Expression Data
Assay Details
Assay
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Reference:
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J:80743
Biffo S, et al., B-50/GAP43 Expression Correlates with Process Outgrowth in the Embryonic Mouse Nervous System. Eur J Neurosci. 1990;2(6):487-499
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5776227
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Gene symbol:
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Gap43
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Gene name:
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growth associated protein 43
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Antibody:
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8613
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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The antibody used in Tables 1 &2 was not detailed; it could have been either #8613 or #6378 MGI:5776190. It was noted that both antibodies gave an identical staining pattern.
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Results
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Specimen
3C:
embryonic day 12.5
(more )
Specimen
3C:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
4E:
embryonic day 9.5
(more )
Specimen
4E:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
4F:
embryonic day 10.5
(more )
Specimen
4F:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: diencephalon marginal layer |
Present |
|
4F
|
|
TS17: dorsal root ganglion |
Present |
|
4F
|
|
TS17: future spinal cord |
Present |
|
4F
|
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TS17: midbrain marginal layer |
Present |
|
4F
|
|
TS17: telencephalon marginal layer |
Present |
|
4F
|
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TS17: hindbrain marginal layer |
Present |
|
4F
|
Expression was in the metencephalon and myelencephalon marginal layers.
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TS17: trigeminal ganglion |
Present |
|
4F
|
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TS17: cranial ganglion |
Present |
Regionally restricted |
4F
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All the cranial nerve associated ganglia (geniculate, auditory, superior, submaxillary, petrosal, jugular, nodose) were positive, with the exception of the ciliary ganglion.
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TS17: autonomic nervous system |
Present |
Regionally restricted |
4F
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Expression was detected in the sympathetic ganglia (sympathetic trunk, superior cervical, celiac, and superior and inferior mesenteric).
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Specimen
5B:
embryonic day 10.5
(more )
Specimen
5B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5D:
embryonic day 10.5
(more )
Specimen
5D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6B:
embryonic day 11.5
(more )
Specimen
6B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6D:
embryonic day 11.5
(more )
Specimen
6D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6F:
embryonic day 11.5
(more )
Specimen
6F:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: olfactory epithelium |
Present |
Scattered |
6F
|
Expression was detected in the olfactory neuroepithelium. Immunoreactive fibers were noted.
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Specimen
7D:
embryonic day 12.5
(more )
Specimen
7D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: medulla oblongata |
Present |
|
7D
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Expression was detected in the medulla oblongata. Longitudinal and transversal fiber tracts show expression.
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Specimen
Table 1 E9.5:
embryonic day 9.5
(more )
Specimen
Table 1 E9.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
Table 1 E10.5:
embryonic day 10.5
(more )
Specimen
Table 1 E10.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
Table 1 E11.5:
embryonic day 11.5
(more )
Specimen
Table 1 E11.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
Table 1 E12.5:
embryonic day 12.5
(more )
Specimen
Table 1 E12.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
Table 1 E13.5:
embryonic day 13.5
(more )
Specimen
Table 1 E13.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
Table 1 E14.5:
embryonic day 14.5
(more )
Specimen
Table 1 E14.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
Table 1 E15.5:
embryonic day 15.5
(more )
Specimen
Table 1 E15.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
not shown:
embryonic day 8.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: embryo |
Absent |
|
|
Expression could not be demonstrated in any presumptive neuronal or non-neuronal structure.
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Specimen
Table 2 E8.5:
embryonic day 8.5
(more )
Specimen
Table 2 E8.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: future telencephalon |
Absent |
|
|
Expression was not detected in the telencephalon primitive vesicle.
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Specimen
Table 2 E9.5:
embryonic day 9.5
(more )
Specimen
Table 2 E9.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: telencephalon |
Moderate |
Regionally restricted |
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Expression was detected in telencephalon primitive vesicle.
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TS15: diencephalon |
Absent |
|
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There was no expression in the diencephalon primitive vesicle.
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TS15: future midbrain |
Strong |
Regionally restricted |
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Expression was strong in the ventral mesencephalon primitive vesicle. There was no staining in dorsal mesencephalon primitive vesicle.
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TS15: future hindbrain |
Strong |
Regionally restricted |
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Expression was strong in the metencephalon primitive vesicle. There was no expression in the myelencephalon primitive vesicle.
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Specimen
Table 2 E10.5:
embryonic day 10.5
(more )
Specimen
Table 2 E10.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: telencephalon |
Strong |
Regionally restricted |
|
Expression was detected in telencephalon primitive vesicle.
|
TS17: diencephalon |
Moderate |
Regionally restricted |
|
Expression was detected in the diencephalon primitive vesicle. There was no expression in the hypothalamus or thalamus.
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TS17: midbrain |
Present |
Regionally restricted |
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Expression was very strong in the ventral mesencephalon primitive vesicle and moderate in dorsal mesencephalon primitive vesicle.
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TS17: hindbrain |
Strong |
Regionally restricted |
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Expression was very strong in the metencephalon primitive vesicle and strong in the myelencephalon primitive vesicle.
|
Specimen
Table 2 E11.5:
embryonic day 11.5
(more )
Specimen
Table 2 E11.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: telencephalon |
Present |
Regionally restricted |
|
Expression was detected in telencephalon primitive vesicle (very strong), olfactory bulb (moderate) and basal forebrain (moderate). There was no expression in the neocortex anlage.
|
TS19: diencephalon |
Present |
Regionally restricted |
|
Expression was moderate in the diencephalon primitive vesicle and thalamus and strong in the hypothalamus.
|
TS19: midbrain |
Very strong |
Regionally restricted |
|
Expression was very strong in the ventral and dorsal mesencephalon primitive vesicle.
|
TS19: hindbrain |
Very strong |
Regionally restricted |
|
Expression was very strong in the metencephalon primitive vesicle and in the myelencephalon primitive vesicle.
|
TS19: metencephalon basal plate |
Moderate |
Regionally restricted |
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Expression was detected in the pons.
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Specimen
Table 2 E12.5:
embryonic day 12.5
(more )
Specimen
Table 2 E12.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: telencephalon |
Strong |
Regionally restricted |
|
Expression was detected in telencephalon primitive vesicle (very strong), olfactory bulb (strong) and basal forebrain (strong).
|
TS20: neocortex |
Moderate |
|
|
|
TS20: diencephalon |
Very strong |
Regionally restricted |
|
Expression was very strong in the diencephalon primitive vesicle.
|
TS20: hypothalamus |
Strong |
|
|
|
TS20: thalamus |
Strong |
|
|
|
TS20: midbrain |
Strong |
Regionally restricted |
|
Expression was very strong in the ventral and dorsal mesencephalon primitive vesicle and strong in the tuberculum posterius.
|
TS20: cerebellum primordium |
Moderate |
|
|
|
TS20: pons |
Strong |
|
|
|
TS20: medulla oblongata |
Strong |
Regionally restricted |
|
Expression was very strong in the metencephalon primitive vesicle and in the myelencephalon primitive vesicle.
|
TS20: metencephalon |
Strong |
Regionally restricted |
|
Expression was very strong in the metencephalon primitive vesicle and in the myelencephalon primitive vesicle.
|
Specimen
Table 2 E13.5:
embryonic day 13.5
(more )
Specimen
Table 2 E13.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
Table 2 E14.5:
embryonic day 14.5
(more )
Specimen
Table 2 E14.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
Table 2 E15.5:
embryonic day 15.5
(more )
Specimen
Table 2 E15.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
2% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|