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Gene Expression Data
Assay Details
Assay
Reference: J:138816 Plusa B, et al., Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst. Development. 2008 Sep;135(18):3081-91
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5776184
Gene symbol: Gata6
Gene name: GATA binding protein 6
Antibody: anti-Gata6
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Heterozygous and homozygous embryos were indistinguishable.
Results
Specimen 1C: embryonic day 4.0; Pdgfratm11(EGFP)Sor/? (more )
Note: Double labeled: red - Gata6; green - Pdgfra reporter (assay MGI:5776158).
Structure Level Pattern Image Note
TS5: inner cell mass Present Regionally restricted 1C merge , 1C GATA6 Expression is localized to the primitive endoderm layer of the blastocyst; it co-localized with a reporter for another gene that is a marker for this layer.
Specimen 3A: embryonic day 3.0; Pdgfratm11(EGFP)Sor/? (more )
Note: 16-cell embryo; double labeled: red - Gata6; green - Pdgfra reporter (assay MGI:5776158).
Structure Level Pattern Image Note
TS3: compacted morula Present 3A merge , 3A Gata6
Specimen not shown - 2-cell embryos: embryonic day 1.0 (more )
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Absent No expression was seen in any of the 17 embryos examined.
Specimen not shown - 4-cell embryos: embryonic day 2.0 (more )
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Absent No expression was seen in 7 of 9 embryos examined.
Specimen not shown - 4-cell embryos: embryonic day 2.0 (more )
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present Expression was seen in 2 of 9 embryos examined.
Specimen 3B: embryonic day 2.5 (more )
Note: 8-cell embryo; double labeled: red - Gata6; white - Nanog (assay MGI:5776486).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 3B Gata6 , 3B merge Expression is seen in the majority of embryos (9 of 11) at this stage.
Specimen 3C: embryonic day 3.5 (more )
Note: 32-cell morula; double labeled: red - Gata6; white - Nanog (assay MGI:5776486).
Structure Level Pattern Image Note
TS4: compacted morula Strong Widespread 3C Gata6 , 3C merge Strong staining is observed in both inside and outside non-dividing cells.
Specimen 3D: embryonic day 3.5 (more )
Note: 33-cell early blastocyst; double labeled: red - Gata6; white - Nanog (assay MGI:5776486).
Structure Level Pattern Image Note
TS4: inner cell mass Present 3D Gata6 , 3D merge 7 embryos were examined.
TS4: trophectoderm Present 3D Gata6 , 3D merge 7 embryos were examined.
Specimen 3E: embryonic day 4.0 (more )
Note: 58-cell blastocyst; double labeled: red - Gata6; white - Nanog (assay MGI:5776486).
Structure Level Pattern Image Note
TS5: blastocyst Present Regionally restricted 3E Gata6 , 3E merge Expression is mutually exclusive of Nanog.
Specimen Table S1 - 8-cell embryos: embryonic day 2.5 (more )
Note: Also stained for Nanog (assay MGI:5776486).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 11 embryos were examined. Expression was seen in 80 of 84 cells examined; 54 (67.5%) of these cells also expressed Nanog.
Specimen Table S1 - 9-15-cell embryos: embryonic day 3.0 (more )
Note: Also stained for Nanog (assay MGI:5776486).
Structure Level Pattern Image Note
TS3: compacted morula Present 9 embryos were examined. Expression was seen in 100 of 115 cells examined; 78 (78%) of these cells also expressed Nanog.
Specimen Table S1 - 16-32-cell embryos: embryonic day 3.5 (more )
Note: Also stained for Nanog (assay MGI:5776486).
Structure Level Pattern Image Note
TS4: compacted morula Present 8 embryos were examined. Expression was seen in 234 of 244 cells examined; 144 (61.5%) of these cells also expressed Nanog.
Specimen Table S1 - 32-64-cell embryos: embryonic day 3.5 (more )
Note: Also stained for Nanog (assay MGI:5776486).
Structure Level Pattern Image Note
TS4: blastocyst Present 8 embryos were examined. Expression was seen in 268 of 319 cells examined; 130 (48.5%) of these cells also expressed Nanog.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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