Probe:	pBSSK-MGF-4
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS19: heart septum	Strong		1A/B
TS19: maxillary process	Strong		1A/B
TS19: neural tube floor plate	Strong		1A/B
TS19: neural tube lateral wall	Strong	Regionally restricted	1A/B	Expressed in the motor columns.
TS19: surface ectoderm	Present		1A/B	Expressed in the skin.
TS19: liver	Present		1A/B
TS19: urogenital ridge	Present		1A/B

Structure	Level	Pattern	Image	Note
TS21: skin	Present		1D/E	Most prominent.
TS21: brain	Present		1D/E
TS21: gonad	Present		1D/E
TS21: metanephros	Present		1D/E
TS21: spinal cord	Present		1D/E

Structure	Level	Pattern	Image	Note
TS16: urogenital ridge	Present		2A/B	Highest level.

Structure	Level	Pattern	Image	Note
TS16: urogenital ridge	Present	Graded	2D	Highest level in the genital ridge, with a decreasing gradient of grains seen toward the vicinity of the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS16: trunk mesenchyme	Present	Graded	2E	Highest level in the genital ridge, with a decreasing gradient of grains seen toward the vicinity of the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS16: trunk mesenchyme	Present	Graded	2F	Highest level in the genital ridge, with a decreasing gradient of grains seen toward the vicinity of the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS15: urogenital ridge	Present		3A/E

Structure	Level	Pattern	Image	Note
TS19: urogenital ridge	Present		3B/F

Structure	Level	Pattern	Image	Note
TS21: renal tubule	Present		3C/G
TS21: testis	Present		3C/G

Structure	Level	Pattern	Image	Note
TS22: ovary	Present		3D/H
TS22: renal tubule	Ambiguous		3D/H	Decreased expression.

Structure	Level	Pattern	Image	Note
TS28: ovary follicle	Present	Regionally restricted	4A/B	Expressed outside the oocytes and at highest levels in the surrounding granulosa cells.

Structure	Level	Pattern	Image	Note
TS21: dermis	Present	Regionally restricted	5A/B , 5D/E	Expressed in the mesenchymal cells underlying the dermis of the forelimb.

Structure	Level	Pattern	Image	Note
TS23: dermis	Present	Regionally restricted	5H	Expressed in the mesenchymal cells underlying the dermis of the hindlimb.

Structure	Level	Pattern	Image	Note
TS23: dermis	Present	Regionally restricted	5K/L	Expressed in the mesenchymal cells underlying the dermis.

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		6A/B

Structure	Level	Pattern	Image	Note
TS20: spinal cord floor plate	Present		6C
TS20: spinal cord lateral wall	Present	Regionally restricted	6C	Expressed along the ventrolateral boundary in the region where the motor neuron columns form.

Structure	Level	Pattern	Image	Note
TS22: spinal cord lateral wall	Present	Regionally restricted	6F/G	Expressed in the neurons of the forming motor columns and in cells of the ventral marginal zone which appear to migrate toward the motor columns.
TS22: spinal cord marginal layer	Present	Regionally restricted	6F/G	Expressed in cells of the ventral marginal zone which appear to migrate toward the motor columns.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain floor plate	Present		7A/B

Structure	Level	Pattern	Image	Note
TS19: midbrain floor plate	Present		7C	Increased expression.

Structure	Level	Pattern	Image	Note
TS21: hindbrain floor plate	Present		7F/G
TS21: hindbrain lateral wall	Present	Regionally restricted	7F/G	Expressed in two groups of cells close to the lateral boundaries of the floor of the myelencephalon.

Structure	Level	Pattern	Image	Note
TS23: thalamus	Strong	Regionally restricted	7I	Expressed in the dorsal thalamus just caudal to the layer of Kit expressing cells.

Structure	Level	Pattern	Image	Note
TS23: olfactory cortex	Strong	Regionally restricted	7L/M	Expressed in punctate cells of the intermediate zone and in the ventricular epithelium.
TS23: olfactory cortex ventricular layer	Strong	Punctate	7L/M

Structure	Level	Pattern	Image	Note
TS25: dorsal root ganglion	Strong		8A/B	Distributed in a larger proportion of cells.

Structure	Level	Pattern	Image	Note
TS14: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present			Expressed throughout the length of the neural tube.

Structure	Level	Pattern	Image	Note
TS19: hindbrain floor plate	Present			Increased expression.
TS19: neural tube floor plate	Present			Increased expression.

Structure	Level	Pattern	Image	Note
TS23: renal tubule	Absent

Structure	Level	Pattern	Image	Note
TS25: liver	Present			Reduced expression.
TS25: skin	Present			Reduced expression.
TS25: brain	Strong
TS25: cranial ganglion	Strong
TS25: spinal cord	Strong
TS25: spinal ganglion	Strong