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Gene Expression Data
Assay Details
Assay
Reference: J:41287 Zhou L, et al., Cloning and expression of a novel murine semaphorin with structural similarity to insect semaphorin I. Mol Cell Neurosci. 1997 Jan;9(1):26-41
Assay type: RNA in situ
MGI Accession ID: MGI:5755885
Gene symbol: Sema3a
Gene name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
Probe: SD88
Probe preparation: Antisense labelled with P32 RNA
Visualized with: Autoradiography
Results
Specimen 6B: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: metencephalon ventricular layer Present 6B Discrete areas of moderate or intense expression in ventricular neuroepithelium
TS21: cerebral cortex Present 6B Expression in the developing cortical layers,
TS21: corpus striatum Present Regionally restricted 6B Expression in the anlage of the caudate/putamen.

Specimen 6D: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: midbrain tegmentum Present Regionally restricted 6D Expression has expanded to include most of the tegmentum.
TS24: pons Ambiguous 6D No expression in the raphe region of the pons.
TS24: hypothalamus Present Regionally restricted 6D In the hypothalamus, expression limited to the arcuate nucleus.
TS24: nasal capsule Present 6D
TS24: olfactory epithelium Present 6D
TS24: nasal septum cartilage Absent 6D

Specimen 7B: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: cerebellum Purkinje cell cluster Strong Regionally restricted 7B Intense but transient expression limited to clusters of migratory and postmigratory Purkinje cells.

Specimen 7D: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: cerebellum Purkinje cell cluster Strong Regionally restricted 7D Intense but transient expression limited to clusters of migratory and postmigratory Purkinje cells.

Specimen 7F: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: cerebellum Purkinje cell cluster Strong Regionally restricted 7F Intense but transient expression limited to clusters of migratory and postmigratory Purkinje cells.

Specimen 8B: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: neural retina Present Regionally restricted 8B Expression in neural retina in retinal ganglion cell layer.
TS24: optic nerve Present Regionally restricted 8B Expression in cells lining the optic nerve.
TS24: perioptic mesenchyme Present 8B Expression in connective tissue surrounding the eye and extraocular muscles.

Specimen 8D: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: lens epithelium Present 8D

Specimen 8F: postnatal adult (more )
Structure Level Pattern Image Note
TS28: eye Absent 8F Authors state that expression in the eye declines after postnatal day 1.

Specimen E11.5 not shown: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: telencephalon Absent
TS19: neural retinal epithelium Present
TS19: lens vesicle Present Regionally restricted Expression in anterior region of lens.

Specimen E15.5 not shown: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: neural retina Present
TS23: lens Present Regionally restricted Expression in anterior region of lens.
TS23: nasal capsule Present

Specimen PN1 not shown: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: cortical plate Present

Specimen PN4 not shown: postnatal day 4 (more )
Structure Level Pattern Image Note
TS28: cerebellum Absent

Specimen P21 not shown: postnatal day 21 (more )
Structure Level Pattern Image Note
TS28: main olfactory bulb Absent


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory