Probe:	SD89
Probe preparation:	Antisense labelled with P32 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS19: trunk sclerotome	Strong		3A	Robust expression in the sclerotome.
TS19: trunk mesenchyme	Present	Regionally restricted	3A	Prominent expression mesenchymal regions ventral and lateral to sclerotome.
TS19: dermomyotome	Ambiguous		3A	Authors state that expression was less intense or absent in dermamyotome and skin.
TS19: surface ectoderm	Ambiguous		3A	Authors state that expression was less intense or absent in dermamyotome and skin.

Structure	Level	Pattern	Image	Note
TS21: forelimb cartilage condensation	Strong		3B	High levels of expression in muscle and skeletal structures.
TS21: forelimb musculature	Strong		3B	High levels of expression in muscle and skeletal structures.
TS21: skin	Ambiguous		3B	Authors state that expression was either absent or present at low levels in skin and viscera.
TS21: visceral organ system	Ambiguous		3B	Authors state that expression was either absent or present at low levels in skin and viscera.

Structure	Level	Pattern	Image	Note
TS24: forelimb skeleton	Ambiguous		3C	Authors state that expression was downregulated in bone.
TS24: forelimb musculature	Weak		3C	Persistent low level expression in muscle.
TS24: lung	Present		3C

Structure	Level	Pattern	Image	Note
TS19: future spinal cord	Present	Widespread	3D	Expression throughout most of spinal cord.
TS19: neural tube basal columns	Strong		3D	High levels of expression in ventral motor neurons.
TS19: neural tube roof plate	Absent		3D	No expression in the dorsal neuroepithelial layer.
TS19: dorsal root ganglion	Strong		3D	High levels of diffuse expression throughout the dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Strong	Regionally restricted	3E	Intense expression in motor pools and ventral neuroepithelium.
TS21: dorsal root ganglion	Strong		3E	High levels of expression throughout the dorsal root ganglia.
TS21: spinal cord pia mater	Strong		3E	Intense expression in pial layer of developing meninges.

Structure	Level	Pattern	Image	Note
TS24: spinal cord	Strong	Regionally restricted	3F	High levels of expression in ventral two-thirds of spinal cord.
TS24: dorsal grey horn	Present		3F	Expression in laminae I-II of the dorsal horn. Less intense expression in deeper dorsal horn laminae.
TS24: spinal cord white matter	Strong	Regionally restricted	3F	Intense expression in a subset of glial cells in white matter.
TS24: dorsal root ganglion	Present		3F	Decreased levels of expression in the dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS27: spinal cord	Present	Diffuse	3G	Diffuse expression in cells throughout the spinal cord.
TS27: dorsal root ganglion	Weak		3G	Low levels of expression persist in the dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS23: brainstem	Moderate	Diffuse	4A	Moderate diffuse expression throughout the forebrain and brainstem.
TS23: forebrain	Moderate	Diffuse	4A	Moderate diffuse expression throughout the forebrain and brainstem.
TS23: brain ventricular layer	Strong	Regionally restricted	4A	Regions of more intense expression in the ventricular neuroepithelium.
TS23: brain	Strong	Regionally restricted	4A	Regions of more intense expression in areas of active cell migration away from the ventricular zone.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Diffuse	4B	Diffuse expression throughout all layers of the cortex.
TS26: basal ganglia	Weak		4B	Lower levels of expression in basal ganglia.
TS26: external capsule	Absent		4B	Expression largely absent from major fiber pathways such as the external capsule.
TS26: diencephalon	Present		4B	Expression in olfactory bulb and nuclei of the diencephalon.
TS26: main olfactory bulb	Present		4B	Expression in olfactory bulb and nuclei of the diencephalon.
TS26: superior colliculus	Strong	Regionally restricted	4B	Particularly intense expression in superior colliculus, more intense dorsally.

Structure	Level	Pattern	Image	Note
TS26: inferior colliculus	Strong	Widespread	4B inset	Particularly intense expression throughout inferior colliculus.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present		4C	Expression in olfactory bulb restricted to glomeruli and mitral cells.
TS28: main olfactory bulb mitral cell layer	Present		4C	Expression in olfactory bulb restricted to glomeruli and mitral cells.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	Present		4D	Expression in olfactory bulb restricted to mitral cells.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex marginal layer	Strong		5A
TS21: cerebral cortex ventricular layer	Absent		5A
TS21: cortical preplate	Absent		5A

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex marginal layer	Strong		5B
TS23: cortical plate	Strong		5B
TS23: cortical subplate	Strong		5B
TS23: cerebral cortex intermediate zone	Moderate		5B
TS23: cerebral cortex ventricular layer	Absent		5B

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present	Ubiquitous	5C	Ubiquitous expression throughout all layers of cortex.

Structure	Level	Pattern	Image	Note
TS21: metencephalon ventricular layer	Present		6A
TS21: hypothalamus	Present		6A
TS21: cerebral cortex	Present		6A	Expression in the primordial cortex.
TS21: telencephalon ventricular layer	Strong	Regionally restricted	6A	Expression in portions of the developing basal ganglia.
TS21: telencephalon	Present	Regionally restricted	6A	Moderate expression apparent throughout the pons. Specific expression in the dorsal pontine tegmentum and marginal migratory stream.

Structure	Level	Pattern	Image	Note
TS24: pons	Moderate	Regionally restricted	6C	Moderate expression apparent throughout the pons. Specific expression in the dorsal pontine tegmentum and marginal migratory stream.
TS24: hypothalamus	Present	Diffuse	6C
TS24: brain nucleus	Present	Regionally restricted	6C	Distinct expression in the arcuate nucleus.
TS24: nasal septum cartilage	Ambiguous		6C	Authors state that expression was downregulated in nasal cartilage.
TS24: olfactory epithelium	Present		6C

Structure	Level	Pattern	Image	Note
TS23: cerebellum external granule cell layer	Present		7A
TS23: hindbrain ventricular layer	Present		7A	Expression in neuroepithelium of the fourth ventricle.

Structure	Level	Pattern	Image	Note
TS25: cerebellum external granule cell layer	Present		7C
TS25: hindbrain ventricular layer	Present		7C	Expression in neuroepithelium of the fourth ventricle.

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Strong	Widespread	7E

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong	Widespread	7G

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Regionally restricted	7H	Specific expression in presumptive glial cells in subcortical regions.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Regionally restricted	7I	Specific expression in presumptive glial cells in subcortical regions.

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present		8A
TS24: optic nerve	Present	Regionally restricted	8A	Expression in non neuronal cells in optic nerve distal to the optic chiasm.

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present		8C

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present		8E
TS28: retina inner nuclear layer	Present		8E

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Absent
TS19: lens vesicle	Present
TS19: retina epithelium	Present

Structure	Level	Pattern	Image	Note
TS23: lens epithelium	Present
TS23: retina epithelium	Present
TS23: nasal septum cartilage	Present

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Absent
TS28: cerebellum	Absent			Authors state that expression was downregulated throughout the cerebellum.