Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | Notes | References

Symbol:	Col1a1tm20(tetO-GFP,-cas9*D10A)Slowe
Name:	collagen, type I, alpha 1; targeted mutation 20, Scott Lowe
MGI ID:	MGI:5755843
Synonyms:	Apcn, Col1a1tm20(RNU6-gRNA:Apc,tetO-GFP,-cas9*D10A)Slowe
Gene:	Col1a1  Location: Chr11:94827050-94843868 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance:	Col1a1tm20(tetO-GFP,-cas9*D10A)Slowe page

Germline Transmission:	Earliest citation of germline transmission: J:222595
Parent Cell Line:	KH2 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Allele Type:    Targeted (Endonuclease, Inducible, Reporter) Inducer:    doxycycline/tetracycline Mutation:    Insertion   Mutation details: RMCE on KH2 cells inserted into the 3'UTR a RNU6 promoter driving expression of a paired guide RNAs targeting murine Apc 12 bp immediately upstream of the NGG protoscpacer-adjacent motif (PAM) sequence followed by a tetracycline response element promoter driving inducible expression of a GFP report gene, IRES sequence, human codon optimized CRISPR-associated endonuclease nickase variant cas9D10A (Cas9n; derived from Streptococcus pyogenes CRISPR associated protein 9 [SpCas9]) and polyadenylation sequence. (J:222595)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:	160 strains or lines available

Original:	J:222595 Dow LE, et al., Inducible in vivo genome editing with CRISPR-Cas9. Nat Biotechnol. 2015 Apr;33(4):390-4
All:	1 reference(s)